蒙古黄芪种子带菌检测及种衣剂处理  

Detection of Fungi in Seeds of Astragalus membranaceus var.mongholicus and Screening of Seed Coatings

作　　者：宋洁 包芳 郑司浩 王浩 樊良帅 曾燕 王继永 SONG Jie;BAO Fang;ZHENG Si-hao;WANG Hao;FAN Liang-shuai;ZENG Yan;WANG Ji-yong(Hebei University of Chinese Medicine,Shijiazhuang 050091,China;China National Traditional Chinese Medicine Co.,Ltd.,Beijing 100195,China;China Traditional Chinese Medicine Seed&Seedling Co.,Ltd.,Beijing 102600,China)

机构地区：[1]河北中医药大学,河北石家庄050091 [2]中国中药有限公司,北京100195 [3]国药种业有限公司,北京102600

出　　处：《中国现代中药》2024年第4期665-671,共7页Modern Chinese Medicine

基　　金：财政部和农业农村部:国家现代农业产业技术体系资助项目。

摘　　要：目的:通过检测蒙古黄芪种子外部携带真菌孢子数和内部携带真菌种类,筛选适合蒙古黄芪种子的专用种衣剂。方法:采用离体平皿法检测不同地区采集的10批蒙古黄芪种子外部真菌孢子数和内部携带真菌种类,对所携带的真菌进行分离、纯化和鉴定;采用发芽盒及马铃薯葡萄糖琼脂培养基平皿培养基法检验4个常用种衣剂组合对蒙古黄芪种子的抑菌效果。结果:不同批次蒙古黄芪种子外部携带真菌孢子负荷量差异有统计学意义(P<0.05),以甘肃省陇西县最多、山西省五寨县最少;蒙古黄芪种子内部主要携带青霉属、链格孢菌属等真菌。不同产地种子适用的种衣剂配方不同。满益佳种衣剂+锐胜种衣剂对甘肃省陇西县蒙古黄芪种子的抑菌效果较佳,但对甘肃省民乐县、青海省互助县、山西省五寨县种子的抑菌效果较差;利农种衣剂+锐胜种衣剂、顶苗新种衣剂+锐胜种衣剂对山西省应县、甘肃省民乐县、青海省互助县蒙古黄芪种子的抑菌效果较佳,但对其他产地种子抑菌效果较差。结论:不同产地黄芪种子适用种衣剂配方不同,研究结果为开发不同产地蒙古黄芪种子专用种衣剂提供了参考。Objective:To screen suitable seed coatings for Astragalus membranaceus var.mongholicus by detecting the external fungal spore count and internal fungal species.Methods:The plate culture method was employed to detect external fungal spore count and internal fungal species in 10 batches of A.membranaceus var.mongholicus seeds collected from different areas.The fungi were isolated and identified.The germination boxes and potato dextrose agar(PDA)were used to examine the antifungal effects of the combinations of four common seed coatings.Results:The count of fungal spores showed significant differences among the seeds from different areas(P<0.05),being the highest on the seeds from Longxi County in Gansu Province and the lowest on the seeds from Wuzhai County in Shanxi Province.Fungi of Penicillium and Alternaria were detected in the seeds.The combinations of seed coatings showed varied effects on the seeds from different areas.Specifically,the seed coating combination of Manyijia+Ruisheng exhibited strong antifungal effect on the seeds from Longxi County in Gansu Province but poor effects on the seeds from Minle County in Qinghai Province and Wuzhai County in Shanxi Province.Linong/Dingmiaoxin+Ruisheng demonstrated strong antifungal effects on the seeds from Yingxian County in Shanxi Province,Minle County in Gansu Province,and Huzhu County in Qinghai Province but limited effects on the seeds from other areas.Conclusion:This study highlights the need for formulating specific seed coating combinations tailored to the seeds of A.membranaceus var.mongholicu in different producing areas.

关 键 词：蒙古黄芪 带菌检测 种衣剂 抑菌效果 

分 类 号：R282.2[医药卫生—中药学]