MTT法用于中药成分细胞毒性和增殖检测的干扰因素及控制措施  

Interference Factors and Countermeasures in MTT Assay for Detection of Chinese Herbal Medicine Components with Cytotoxicity and Influencing Cell Proliferation

作　　者：褚皓天 赵鹏 田燕歌 任周新[1,2] CHU Hao-tian;ZHAO Peng;TIAN Yan-ge;REN Zhou-xin(Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases Co-constructed by Henan Province&Education Ministry of P.R.China,Zhengzhou 450046,China;Academy of Chinese Medical Sciences,Henan University of Chinese Medicine,Zhengzhou 450046,China)

机构地区：[1]呼吸疾病中医药防治省部共建协同创新中心,河南郑州450046 [2]河南中医药大学中医药科学院,河南郑州450046

出　　处：《中国现代中药》2024年第4期749-756,共8页Modern Chinese Medicine

基　　金：国家自然科学基金项目(82074406);国家中医临床研究基地业务建设第二批科研专项项目(JDZX2015l56)。

摘　　要：3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐(MTT)法具有操作简单和成本低廉等优点,被广泛应用于中药成分的细胞毒性和对细胞增殖影响等研究。然而,细胞的数量和密度、MTT浓度和作用时间等实验条件会干扰检测结果的准确性。另外,由于中药成分种类繁多而且化学结构复杂,可能导致吸收光谱漂移、形成近似检测波长的吸收峰及干扰细胞代谢的活性等,影响MTT检测结果的准确性。对近年的相关文献进行整理,归纳和分析了影响MTT实验的实验条件、优化措施和质量控制,归类了检测需要注意的某些中药成分,总结了替代MTT法的其他实验及MTT与其他实验的联合应用,对应用中存在的问题进行了探讨和展望,期望为MTT法在中药成分相关实验中的合理应用提供参考。The methyl thiazolyl tetrazolium(MTT)assay with simple operation and low cost has been widely used in the research on the Chinese herbal medicine components which have cytotoxicity and influence cell proliferation.However,the test conditions such as the number and density of cells,MTT concentration,and reaction duration interfere with the accuracy of the test results.In addition,the diverse components with complex structures in Chinese herbal medicines may affect the accuracy of the detection results due to the drift of the absorption spectrum,interference of cellular metabolic activity,absorption peaks of approximate detection wavelengths,which result in erroneous conclusions.By reviewing the relevant articles published in recent years,the paper summarizes and analyzes the interference factors in test conditions and corresponding optimization measures and quality control measures.Furthermore,this paper classifies some Chinese herbal medicine components that need attention in the assay,summarizes the alternative methods of MTT assay and the application of MTT assay combined with other assays,and discusses the existing problems in the application,aiming to provide reference for the rational application of MTT assay in the research on Chinese herbal medicine components.

关 键 词：干扰因素 中草药成分 3-(4 5-二甲基噻唑-2)-2 5-二苯基四氮唑溴盐法 优化措施 替代方法 

分 类 号：R285[医药卫生—中药学]