bFGF通过NF-κB信号通路抑制人子宫平滑肌收缩  

bFGF can inhibit contraction of human uterine smooth muscle through NF-κB signaling pathway.

作　　者：都园渊 朱盛兰 张慧婷 江一 周璇[1] 丁文成[1] 冯玲[1] Du Yuanyuan;Zhu Shenglan;Zhang Huiting(Department of Obstetrics and Gynecology,Tongji Hospital,Tongji Medical College,Huazhong University of Science&Technology,Wuhan 430000)

机构地区：[1]华中科技大学同济医学院附属同济医院妇产科,武汉430000

出　　处：《现代妇产科进展》2024年第5期357-361,368,共6页Progress in Obstetrics and Gynecology

基　　金：国家重点研发计划(No:2021YFC2701502)。

摘　　要：目的:探讨外源性碱性成纤维细胞生长因子(bFGF)对人子宫平滑肌原代细胞(HUt SMCs)收缩功能的影响及其可能机制。方法:用不同浓度外源性bFGF对子宫平滑肌细胞进行干预,qRT-PCR法检测缝隙连接蛋白(CX43)、催产素受体(OXTR)mRNA水平,Western blot法检测CX43、OXTR蛋白表达;胶原收缩实验研究外源性bFGF对HUt SMCs收缩能力的影响;激光共聚焦显微镜观察细胞内Ca 2+水平。用外源性bFGF和JSH-23(NF-κB转录活性抑制剂)处理人子宫平滑肌原代细胞,Western blot法检测CX43和NF-κB p65蛋白表达;酶联免疫吸附实验(ELISA)检测HUt SMCs分泌的IL-6、IL-8水平。结果:bFGF可抑制HUt SMCs收缩相关分子(CX43、OXTR)mRNA和蛋白表达。bFGF可抑制HUt SMCs收缩能力并使细胞内Ca 2+水平降低。bFGF上调人子宫平滑肌细胞NF-κB蛋白表达并下调CX43蛋白表达,促进其分泌IL-8,这种效应被JSH-23所逆转。结论:bFGF通过激活NF-κB通路发挥促炎作用并调控CX43表达,从而参与调节HUt SMCs的收缩能力。Objective:To investigate the effect of exogenous basic fibroblast growth factor(bFGF)on contractility of human uterine smooth muscle cells(HUt SMCs)and its possible mechanism.Methods:Exogenous bFGF of different concentrations were used to treat HUt SMCs.The mRNA levels of connexin 43(CX43)and oxytocin receptor(OXTR)were detected by qRT-PCR,and the expressions of CX43 and OXTR proteins were detected by Western blot.Collagen contractile test was used to study the effect of exogenous bFGF on the contractility of HUt SMCs.The level of intracellular Ca 2+was observed by laser confocal microscope.HUt SMCs were treated with exogenous bFGF and JSH-23(NF-κB transcription activity inhibitor),and the expression of CX43 was detected.ELISA was used to detect the levels of IL-6 and IL-8 secreted by HUt SMCs.Results:bFGF could inhibit the expression of contraction-related molecules(CX43,OXTR)mRNA and protein in HUt SMCs.bFGF can inhibit the contractility of HUt SMCs and decrease the concentration of intracellular Ca 2+.bFGF upregulates the expression of key proteins of NF-κB pathway and downregulates the expression of CX43 and NF-κB p65 protein.This effect is reversed by JSH-23.Conclusion:bFGF plays a pro-inflammatory role by activating NF-κB pathway and regulating the expression of CX43,which involves in regulating the contractility of HUt SMCs.

关 键 词：碱性成纤维细胞生长因子 NF-ΚB通路 宫缩乏力 产后出血 

分 类 号：R711.74[医药卫生—妇产科学]