黄芪多糖对黄喉拟水龟生长性能、血清生化指标及肠道菌群结构的影响  

Effects of Astragalus polysaccharides on growth performance,serum biochemical indexes and intestinal flora structure of Mauremys mutica

作　　者：吴志刚 张洪耀 高铭佐 严子科 谭雯予 韩书煜 施金谷 黄德生 梁静真 黄钧 WU Zhi-gang;ZHANG Hong-yao;GAO Ming-zuo;YAN Zi-ke;TAN Wen-yu;HAN Shu-yu;SHI Jin-gu;HUANG De-sheng;LIANG Jing-zhen;HUANG Jun(College of Animal Science and Technology,Guangxi University/Guangxi Laboratory of Aquatic Animal Disease Diagnosis/Guangxi University Key Laboratory of Aquatic Healthy Breeding and Nutrition Regulation,Nanning,Guangxi 530004,China;Guangxi Station of Aquaculture Technology Extension,Nanning,Guangxi 530022,China;Hepu Station of Aquaculture Technology Extension,Beihai,Guangxi 536199,China)

机构地区：[1]广西大学动物科学技术学院/广西水生动物病害诊断实验室/广西高校水生生物健康养殖与营养调控重点实验室,广西南宁530004 [2]广西水产技术推广站,广西南宁530022 [3]合浦县水产技术推广站,广西北海536199

出　　处：《南方农业学报》2024年第2期388-396,共9页Journal of Southern Agriculture

基　　金：国家自然科学基金项目(202100475);广西自然科学基金项目(2018GXNSFAA138167)。

摘　　要：【目的】探究饲料中添加不同水平的黄芪多糖对黄喉拟水龟(Mauremys mutica)生长性能、血清生化指标和肠道菌群结构的影响,以期为黄芪多糖在黄喉拟水龟饲料中的应用及黄喉拟水龟的健康养殖提供理论依据。【方法】将初始平均体重为128.36±1.80g的黄喉拟水龟随机分为5组,分别投喂黄芪多糖含量为0(M0,对照组)、0.25%(M25)、0.50%(M50)、0.75%(M75)和1.00%(M100)的等氮等脂饲料,每组设3个重复,每重复10只龟,投喂60 d后测定其生长性能指标和血清生化指标,以高通量测序分析肠道菌群结构。【结果】生长性能分析结果显示,M25、M50和M75组的黄喉拟水龟的增重率和特定生长率显著高于M0组(P<0.05,下同),饲料系数显著低于M0组,其中M50组的增重率和特定生长率最高、饲料系数最低。M25、M50、M75和M100组黄喉拟水龟背甲长、背甲宽和背甲高的日增长与M0组比较均无显著差异(P>0.05,下同)。血清生化指标检测结果显示,M25、M50和M75组的碱性磷酸酶和超氧化物歧化酶活性显著高于M0组,M50、M75和M100组的过氧化氢酶活性显著高于M0组;M25、M50和M75组的丙二醛含量显著降低。肠道菌群分析结果显示,M25组群落丰富度指数(Sobs指数和ACE指数)显著高于M0组,群落多样性指数(Shannon指数和Simpson指数)与M0组无显著差异。M50、M75和M100组的Sobs指数、Chao1指数、ACE指数、Shannon指数和Simpson指数与M0组差异均不显著。在门分类水平上,各组丰度占比排名前3的菌群均为厚壁菌门、拟杆菌门和变形菌门,0.25%~1.00%的黄芪多糖对门分类水平的肠道菌群组成无显著影响。在属分类水平上,M50组的瘤胃球菌属1和M75组的汉氏盐单胞菌属相对丰度显著提高;添加0.25%和1.00%的黄芪多糖对属分类水平的肠道菌群组成均无显著影响。【结论】饲料中添加适量的黄芪多糖能有效地促进黄喉拟水龟生长、提高抗氧化能力并改善肠道�【Objective】To investigate the effects of different levels of Astragalus polysaccharides on the growth perfor‐mance,serum biochemical indexes and intestinal flora structure of Mauremys mutica,with a view to providing theoretical basis for the application of Astragalus polysaccharides in the diets of M.mutica and the healthy breeding of M.mutica.【Method】M.mutica with an initial average body weight of 128.36±1.80 g were randomly divided into five groups,and fed isonitrogenous and isofat diets containing 0(M0),0.25%(M25),0.50%(M50),0.75%(M75),and 1.00%(M100)Astragalus polysaccharides,with three replicates of 10 M.mutica in each group.After feeding for 60 d,the growth perfor‐mance indexes and serum biochemical indexes were measured,and the structure of intestinal flora was analyzed by highthroughput sequencing.【Result】The results of growth performance analysis showed that the weight gain rate and specific growth rate of M.mutica in the M25,M50 and M75 groups were significantly higher than those in the M0 group(P<0.05,the same below),and the feed coefficients were significantly lower than those in the M0 group,among which the M50 group had the highest weight gain rate and specific growth rate,and the lowest feed coefficient,and the daily growth rates of the dorsal carapace length,dorsal carapace width and dorsal carapace height of M.mutica in the M25,M50,M75 and M100 groups had no significant difference from those of the M0 group(P>0.05,the same below).The results of serum biochemical indexes showed that the activities of alkaline phosphatase and superoxide dismutase in M25,M50 and M75 groups were significantly higher than that of M0 group,and the activities of catalase in M50,M75 and M100 groups were significantly higher than that of M0 group;and the contents of malondialdehyde in M25,M50 and M75 groups were significantly lower.The results of intestinal flora analysis showed that the community richness indexes(Sobs index and ACE index)were significantly higher in M25 group than in M0 group,and the com

关 键 词：黄芪多糖 黄喉拟水龟 生长性能 血清生化指标 肠道菌群结构 

分 类 号：S966.5[农业科学—水产养殖]