微小RNA-26a-5p对氧化型低密度脂蛋白处理的血管平滑肌细胞增殖、迁移和泡沫化的影响  

Effects of microRNA-26a-5p on proliferation,migration and bubbling of vascular smooth muscle cells treated with oxidized low-density lipoprotein

作　　者：赵妙惠[1] 周佳妮[1] 吴超群 ZHAO Miaohui;ZHOU Jiani;WU Chaoqun(Ningbo Medical Center Li Huili Hospital,Ningbo 315040,Zhejiang,China)

机构地区：[1]宁波市医疗中心李惠利医院,宁波315040

出　　处：《现代实用医学》2024年第4期428-432,共5页Modern Practical Medicine

基　　金：宁波市科技计划项目(202003N4226)。

摘　　要：目的探究微小RNA-26a-5p(miR-26a-5p)对动脉粥样硬化(AS)过程中血管平滑肌细胞(VSMCs)增殖、迁移和泡沫化的影响。方法使用氧化型低密度脂蛋白(ox-LDL)处理VSMCs,模拟AS过程中的VSMCs细胞模型,向细胞中转染miR-26a-5p模拟物(miR-26a-5p mimic)及其阴性对照(NC mimic),将细胞分为对照组、ox-LDL组、ox-LDL+NC mimic组、ox-LDL+miR-26a-5p mimic组。通过qRT-PCR检测细胞miR-26a-5p表达水平,采用CCK-8和EDU染色检测细胞活力和增殖,通过划痕实验和Transwell实验检测细胞迁移和侵袭能力,使用油红O染色观察细胞中脂滴聚集情况,采用免疫荧光实验检测细胞中α-SMA的表达。结果VSMCs中miR-26a-5p水平随着ox-LDL浓度的增加和作用时间的延长而降低。与对照组比较,ox-LDL组VSMCs细胞增殖、迁移、侵袭能力显著增强,脂滴聚集增多,α-SMA信号减弱,泡沫化程度增强。与oxLDL+NC mimic组比较,ox-LDL+miR-26a-5p mimic组细胞增殖、迁移、侵袭能力显著减弱,脂滴聚集减少,α-SMA信号增强,泡沫化程度减弱。结论过表达miR-26a-5p可以下调血管平滑肌细胞的增殖、迁移和泡沫化,miR-26a-5p可能成为AS治疗的新靶点。Objective To investigate the effect of microRNA-26a-5p on vascular smooth muscle cells(VSMCs)proliferation,migration and foaminess during atherosclerosis(AS).Methods VSMCs were treated with oxidized low-density lipoprotein(ox-LDL)to construct a VSMCs model during AS simulation.The miR-26a-5p mimic and its negative control mimic(NC mimic)were transfected into the cells,and the cells were divided into the control group,ox-LDL group,ox-LDL+NC mimic group,and ox-LDL+miR-26a-5p mimic group.Quantitative real time-polymerase chain reaction(qRT-PCR)was used to detect the expression of miR-26a-5p.Cell Countingkit-8(CCK-8)and 5-ethynyl-2'-deoxyuridine(EDU)staining were used to assess cell viability.Transwell and wound healing assay was used to analyze cell migration,and oil red O staining(ORO)was performed to observe the lipid droplet accumulation in cells.Immunofluorescence(IF)assay was used to observe the-SMA expression in cells.Results The miR-26a-5p level in VSMCs decreased with increasing ox-LDL treatment concentration and time.Compared with the control group,the proliferation,migration,and invasion ability of VSMCs cells in the ox-LDL group were significantly enhanced,and lipid droplet aggregation increased,-SMA signal weakened and foam degree increased.The proliferation,migration and foaminess were up-regulated by ox-LDL,while miR-26a-5p mimic effectively reversed these changes.Conclusions Over-expression of miR-26a-5p downregulates proliferation,migration and foaming of VSMCs,and miR-26a-5p may be a new target for AS therapy.

关 键 词：动脉粥样硬化 miR-26a-5p OX-LDL VSMCS 泡沫细胞 

分 类 号：R543[医药卫生—心血管疾病]