预防禽类呼吸道疾病的中药固体空气清新剂研制和抑菌效果分析  

Preparation and Antibacterial Effects Analysis of Traditional Chinese Medicine Solid Air Freshener for Preventing Avian Respiratory Diseases

作　　者：唐豫媛 周晓彤 包凤珠 高小康 TANG Yuyuan;ZHOU Xiaotong;BAO Fengzhu;GAO Xiaokang(College of Pharmacy,Hubei University of Medicine,Shiyan 442000,China;Hubei Key Laboratory of Wudang Characteristic Traditional Chinese Medicine,Shiyan 442000,China;Taihe Hospital Stem Cell Research Centre,Shiyan 442000,China)

机构地区：[1]湖北医药学院药学院,湖北十堰442000 [2]武当特色中药研究湖北省重点实验室,湖北十堰442000 [3]湖北省太和医院干细胞研究中心,湖北十堰442000

出　　处：《中国兽医杂志》2024年第5期125-131,共7页Chinese Journal of Veterinary Medicine

基　　金：十堰市科技局一般项目(19K68);湖北省中央引导地方专项基金(2019ZYYD071);湖北医药学院校级人才启动金(K12873)。

摘　　要：为研制一款可净化空气、预防禽类呼吸道疾病且使用方便的中药固体空气清新剂,本试验根据国医大师周仲瑛的中药防疫香囊配方,提取广藿香、苍术、白芷、草果、石菖蒲和艾叶混合后药材的挥发油,添加增溶剂和乳化剂制成中药固体空气清新剂。将其置于室温检测挥发量,以琼脂扩散法考察含不同挥发油的固体空气清新剂以及不同挥发程度的混合药材固体空气清新剂对引起呼吸道疾病的致病菌(大肠杆菌、金黄色葡萄球菌和白色葡萄球菌)的抑菌效果,根据抑菌圈直径的大小评价固体空气清新剂的抑菌活性。结果显示:固体空气清新剂的组成为卡拉胶(20%)、苯甲酸(0.2%)、挥发油(4%)、聚山梨醇酯(1%)和蒸馏水;50 g固体空气清新剂的挥发性较好;含混合药材挥发油的固体空气清新剂对大肠杆菌无明显抑菌效果,对金黄色葡萄球菌和白色葡萄球菌的抑菌圈直径分别为20.1和41.7 mm,属于高敏;同时该固体空气清新剂挥发不同程度后的固体清新剂仍能抑制金黄色葡萄球菌和白色葡萄球菌的生长,说明所制备的混合药材固体空气清新剂能持续对这2种致病菌的生长起到抑制作用。结果表明,该中药固体空气清新剂制备工艺简单,气味芳香怡人,具有净化空气和抑制致病菌生长的功效,能对禽类呼吸道疾病的传播进行有效预防。In order to develop a convenient-to-use traditional Chinese medicine solid air freshener that can purify the air and prevent avian respiratory diseases,this study extracted the volatile oils of herbs including Pogostemon cablin,Atractylodes macrocephala,Angelica dahurica,Fructus tsaoko,Acorus calamus,and Artemisia argyi mixed according to the prescription of the traditional Chinese medicine master Zhou Zhongying.The volatile oils were added with a solubilizer and emulsifier to make the traditional Chinese medicine solid air freshener.The volatile amount of the air freshener was measured at room temperature.The solid air fresheners containing different volatile oils and mixed herb solid air fresheners with different degrees of volatility were tested for their antibacterial effects on pathogenic bacteria(Escherichia coli,Staphylococcus aureus,and Staphylococcus albus)causing respiratory diseases using the agar diffusion method.The antibacterial activity of the solid air freshener was evaluated based on the inhibition zone diameter.The results showed that the composition of the solid air freshener was carrageenan(20%),benzoic acid(0.2%),volatile oil(4%),polysorbate(1%),and distilled water.The volatile performance of 50 g solid air freshener was good.The solid air freshener containing volatile oils from mixed herbs showed no significant inhibitory effect on Escherichia coli,but it exhibited inhibition zone diameters of 20.1 mm and 41.7 mm against Staphylococcus aureus and Staphylococcus albus,respectively,indicating high sensitivity.Moreover,the solid air freshener with different degrees of volatility still inhibited the growth of Staphylococcus aureus and Staphylococcus albus after volatilization,indicating that the prepared mixed herb solid air freshener could continuously inhibit the growth of these two pathogenic bacteria.The results indicate that the preparation process of this traditional Chinese medicine solid air freshener is simple,and it has a pleasant aroma,effectively purifying the air and inhibiting the

关 键 词：禽类 中药挥发油 固体空气清新剂 呼吸道疾病 预防 

分 类 号：S858.3[农业科学—临床兽医学]