针刺对脑缺血再灌注损伤大鼠海马组织miR-29a-3p、miR-29b-3p表达的影响  

Effect of acupuncture on the expression of miR-29a-3p and miR-29b-3p in hippocampal tissue of rats with cerebral ischemia-reperfusion injury

作　　者：王瑶 江姗姗 谢灿明 向静[1] 唐红 汪红娟 李展富 刘晓月 陈楚淘[1] 田浩梅[1] WANG Yao;JIANG Shan-shan;XIE Can-ming;XIANG Jing;TANG Hong;WANG Hong-juan;LI Zhan-fu;LIU Xiaoyue;CHEN Chu-tao;TIAN Hao-mei(Hunan University of Chinese Medicine,Changsha Hunan 410208,China)

机构地区：[1]湖南中医药大学针灸推拿与康复学院,湖南长沙410208

出　　处：《时珍国医国药》2024年第2期485-488,共4页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(82274662);湖南省自然科学基金项目(2021JJ30490);湖南省长沙市科技局自然科学基金项目(kq2014094);湖南省研究生科研创新项目(2022CX103)。

摘　　要：目的观察针刺干预72 h后脑缺血再灌注损伤(CIRI)大鼠缺血侧海马区miR-29a-3p、miR-29b-3p的表达,探讨针刺促CIRI修复的机制。方法30只SD大鼠随机分为假手术组、模型组和针刺组,每组10只,造模组大鼠使用线栓法制备CIRI模型,假手术组只剥离血管不插入线栓;假手术组和模型组只捆绑不针刺,针刺组捆绑后针刺“大椎”“百会”“水沟”穴,一天治疗两次,共治疗7次。采用Garcia评分法对神经功能进行评定,TTC染色法检测脑梗死面积比,qRT-PCR法检测缺血侧海马区miR-29a-3p、miR-29b-3p的表达。结果干预前与假手术组比较,造模组Garcia评分显著降低(P<0.01),脑梗死面积比显著增加(P<0.01),患侧海马区miR-29a-3p、miR-29b-3p表达量降低(P<0.05,P<0.01);干预72h后与模型组比较,针刺组Garcia评分明显上升(P<0.01),脑梗死面积比显著下降(P<0.01),患侧海马组织miR-29a-3p、miR-29b-3p的表达量明显增加(P<0.01)。结论针刺可减少CIRI大鼠缺血侧脑梗死面积、改善神经功能损伤、发挥脑保护作用,可能与干预72h后上调缺血侧海马区miR-29a-3p、miR-29b-3p表达有关。Objective To observe the expression of miR-29a-3p and miR-29b-3p in the hippocampal region of the ischemic side of rats with cerebral ischemia-reperfusion injury(CIRI)after 72 h of acupuncture intervention,and to explore the mechanism of acupuncture to promote anti-CIRI repair.Methods Thirty SD rats were randomized to sham-operated group,model group and acupuncture group,10 rats in each group,the modeling group was prepared using the thread bolus method,the sham-operated group only stripped the blood vessels without inserting the thread bolus;the sham-operated group and model group were only tied without acupuncture,the acupuncture group was tied and then acupuncture was applied to"Dazhui","Baiji"and"Shuigu".In the sham-operated group and the model group,only the vessels were stripped and no needles were inserted.Garcia's score was used to assess neurological function,TTC staining was used to detect the ratio of cerebral infarct area,and qRT-PCR was used to detect the expression of miR-29a-3p and miR-29b-3p in the hippocampus on the ischemic side.Results Compared with the sham-operated group,the Garcia score was significantly lower(P<0.01),the cerebral infarct area ratio was significantly increased(P<0.01),and the expression of miR-29a-3p and miR-29b-3p in the hippocampal region on the affected side was significantly lower(P<0.01)in the model and needle groups;compared with the model group,the Garcia score was significantly higher(P<0.01)in the needle group,the cerebral infarct area ratio was significantly decreased(P<0.01),and the expression of miR-29a-3p and miR-29b-3p in the affected hippocampal tissue was significantly increased(P<0.01).Conclusion Acupuncture can reduce the area of cerebral infarction on the ischemic side,improve neurological impairment and exert cerebral protective effects in CIRI rats,which may be related to the upregulation of miR-29a-3p and miR-29b-3p expression in the hippocampal region on the ischemic side after 72 h of intervention.

关 键 词：针刺 脑缺血再灌注损伤 miR-29a-3p miR-29b-3p 

分 类 号：R285.5[医药卫生—中药学]