电针肾俞穴对肾阳虚近视豚鼠视网膜M1受体表达的影响  

Effect of Electroacupuncture at Shenshu Point on Expression of M1 Receptor in Retina of Guinea Pigs with Kidney Yang Deficiency

作　　者：张金木 刘冬梅[2] 朱聪 何星伯 蒋文君 郭滨[1,4] Myopia ZHANG Jinmu;LIU Dongmei;ZHU Cong;HE Xingbo;JIANG Wenjun;GUO Bin(Shandong University of Traditional Chinese hina;Eye Hospital Affiliated to Shandong University of Traditional Chinese Medicine,Jinan 250002,China;Shandong Academy of Eye Disease Prevention and Therapy/Shandong Provincial Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Eye Disease,Jinan 250002,China;Postdoctoral Station of Shandong University of Traditional Chinese Medicine,Jinan 250355,China)

机构地区：[1]山东中医药大学,山东济南250355 [2]山东中医药大学附属眼科医院,山东济南250002 [3]山东省眼病防治研究院/山东省中西医结合眼病防治重点实验室,山东济南250002 [4]山东中医药大学博士后流动站,山东济南250355

出　　处：《山东中医药大学学报》2024年第3期316-325,共10页Journal of Shandong University of Traditional Chinese Medicine

基　　金：国家重点研发计划项目(编号:2021YFC2702103);山东省自然科学基金面上项目(编号:ZR2020MH393);山东省博士后创新项目(编号:202101012);中国博士后面上项目(编号:2020M672127)。

摘　　要：目的:观察电针肾俞穴对肾阳虚近视豚鼠生长发育、视力及视网膜毒蕈碱样乙酰胆碱(mAChR)M1受体表达的影响,为肾阳虚近视的治疗提供依据。方法:将60只豚鼠随机分为正常对照(NC)组、透镜诱导近视(LIM)组、肾阳虚近视假穴(KYDM+SHAM)组、肾阳虚近视电针(KYDM+EA)组,每组15只。NC组不采取干预措施;LIM组豚鼠右眼配戴-6.00 D透镜构建近视模型;其余两组在戴镜基础上连续2周腹腔注射氢化可的松(剂量10 mg/kg)构建肾阳虚近视模型,造模2周后,KYDM+EA组电针豚鼠双侧肾俞穴,KYDM+SHAM组电针豚鼠臀部假穴,共4周。观察造模不同时间各组豚鼠一般情况,测量体质量和肛温,检测双眼屈光度和眼轴长度,检测血清游离三碘甲状腺原氨酸(FT3)、游离甲状腺素(FT4)、睾酮(T)、雌二醇(E_(2)),并在造模结束后采用定量聚合酶链反应(Q-PCR)、免疫组织化学和蛋白质印迹法检测视网膜M1受体mRNA和蛋白表达水平。结果:造模2周后,KYDM+SHAM组和KYDM+EA组豚鼠出现毛发枯槁、无光泽及消瘦、拱背眯眼、倦怠懒动、畏寒肢冷等肾阳虚症状,血清FT3、FT4、T水平低于LIM组,E_(2)高于LIM组,体质量、肛温以及右眼屈光度均低于LIM组,眼轴长度长于LIM组,差异均有统计学意义(P<0.05)。电针4周后,KYDM+EA组豚鼠肾阳虚症状有效改善,血清激素水平趋于正常,与KYDM+SHAM组相比,体质量、肛温、右眼屈光度均明显升高,右眼眼轴长度缩短,视网膜M1受体mRNA和蛋白表达水平降低,差异均有统计学意义(P<0.05)。与LIM组相比,KYDM+EA组豚鼠体质量增加,差异有统计学意义(P<0.05);肛温、右眼屈光度和眼轴长度、视网膜M1受体mRNA和蛋白表达水平差异均无统计学意义(P>0.05)。结论:电针肾俞穴有利于促进肾阳虚近视豚鼠生长发育恢复及视力改善,机制可能与降低视网膜中M1受体表达有关。Objective:To observe the effect of electroacupuncture at Shenshu point(BL23)on the growth,vision and expression of retinal muscarinic acetylcholine(mAChR)M1 receptor in guinea pigs with kidney yang deficiency myopia(KYDM),so as to provide a basis for the treatment of kidney yang deficiency myopia.Methods:Total 60 guinea pigs were randomly divided into normal control(NC)group,lens-induced myopia(LIM)group,KYDM+sham acupoint(KYDM+SHAM)group and KYDM+electroacupuncture(KYDM+EA)group,with 15 guinea pigs in each group.The NC group did not take any intervention measures.The LIM group was given a lens of-6.00 D in the right eye to construct the myopia model.The other two groups were given intraperitoneal injection of hydrocortisone(10 mg/kg)for 2 weeks on the basis of wearing glasses to construct the model of kidney yang deficiency myopia.After 2 weeks of modeling,the guinea pigs in the KYDM+EA group received electroacupuncture at bilateral Shenshu points,and the guinea pigs in the KYDM+SHAM group received electroacupuncture at the sham points of the buttocks for 4 weeks.During the modeling period,the general conditions of the guinea pigs in each group were observed,body weight and anal temperature were measured,binocular refraction and axial length were detected,serum free triiodothyronine(FT3),free thyroxine(FT4),testosterone(T)and estradiol(E_(2))were detected,and the mRNA and protein expression levels of M1 receptor in the retina were detected by quantitative polymerase chain reaction(Q-PCR),immunohistochemistry,and Western blot after the end of modeling.Results:After 2 weeks of modeling,the guinea pigs in the KYDM+SHAM group and the KYDM+EA group showed symptoms of kidney yang deficiency,such as hair loss,lack of luster and emaciation,arched back and squinting eyes,tiredness and laziness,intolerance of cold and cold limbs,and the serum FT3,FT4 and T levels were lower than those in the LIM group,and the E_(2) level was higher than that in the LIM group.The body weight,anal temperature,and right eye refraction were l

关 键 词：电针 肾俞穴 肾阳虚 近视 毒蕈碱样乙酰胆碱M1受体 豚鼠 

分 类 号：R289.1[医药卫生—方剂学]