巨刺阳陵泉穴对MCAO模型大鼠运动功能作用机制研究  

Mechanism Study on Effect of Opposing Needling at Yanglingquan Point on Motor Function in Model Rats with Middle Cerebral Artery Occlusion

作　　者：谢心悦 潘彦舒 方琪 王浩 刘含之 王安琪 江洋 孙树勇 粆香 柯雨露 邹忆怀[2] XIE Xinyue;PAN Yanshu;FANG Qi;WANG Hao;LIU Hanzhi;WANG Anqi;JIANG Yang;SUN Shuyong;CHAO Xiang;KE Yulu;ZOU Yihuai(College of Traditional Chinese Medicine,Beijing University of Chinese Medicine,Beijing 100029,China;Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing 100007,China)

机构地区：[1]北京中医药大学中医学院,北京100029 [2]北京中医药大学东直门医院,北京100007

出　　处：《山东中医药大学学报》2024年第3期326-336,共11页Journal of Shandong University of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(编号:82174331);北京中医药大学科研纵向发展基金项目(编号:2019-ZXFZJJ-064)。

摘　　要：目的:观察巨刺阳陵泉穴对大脑中动脉栓塞(MCAO)模型大鼠运动功能障碍的疗效和对胼胝体髓鞘碱性蛋白(MBP)的影响,探讨巨刺阳陵泉穴改善脑梗死运动障碍的机制。方法:将68只雄性SD大鼠随机分为假手术组、模型组、巨刺组和非经非穴组,每组17只。除假手术组外,其余三组均用线栓法制备右侧MCAO模型,术后第2天开始干预,巨刺组电针刺激右侧阳陵泉,非经非穴组电针刺激双侧胁下非经非穴位置,每天1次,每次20 min,连续14 d。测定大鼠抓力、神经功能缺损得分;2,3,5-三苯基氯化四氮唑(TTC)染色计算脑梗死体积百分比,苏木精-伊红染色法(HE)观察胼胝体组织形态,免疫组化、酶联免疫吸附测定(ELISA)法、蛋白质印迹(WB)法、反转录聚合酶链反应(RT-PCR)法分别检测右脑胼胝体MBP蛋白及mRNA表达水平。结果:与假手术组相比,模型组大鼠抓力下降,神经功能缺损得分增加,脑梗死体积百分比增加,胼胝体髓鞘结构崩解,右脑胼胝体MBP蛋白及mRNA表达水平降低,差异均有统计学意义(P<0.05)。与模型组、非经非穴组比,巨刺组大鼠抓力增大,神经功能缺损得分降低,脑梗死体积百分比降低,髓鞘结构较为完整,右脑胼胝体MBP蛋白及mRNA表达水平升高,差异均有统计学意义(P<0.05)。结论:巨刺阳陵泉穴可能通过提高胼胝体MBP蛋白表达水平促进MCAO大鼠运动功能康复。Objective:To observe the therapeutic effect of opposing needling at Yanglingquan acupoint(GB34)on motor dysfunction and the effect on callus myelin basic protein(MBP)in model rats of middle cerebral artery occlusion(MCAO),and to explore the mechanism of opposing needling at Yanglingquan acupoint to improve motor dysfunction of cerebral infarction.Methods:Total 68 male SD rats were randomly divided into sham operation group,model group,opposing needling group and non-acupoint group,with 17 rats in each group.Except for the sham operation group,rats in the other three groups were given thread embolization to prepare model rats with right MCAO.The intervention began on the second day after the operation.The opposing needling group was given electroacupuncture stimulation at right Yanglingquan acupoint,and the non-acupoint group was given electroacupuncture stimulation at bilateral non-acupoint under the lower abdomen,once a day,20 min each time,for 14 consecutive days.The grip strength and neurological deficit scores of rats were measured.The percentage of cerebral infarction volume was calculated by using 2,3,5-triphenyltetrazolium chloride(TTC)staining,the morphology of corpus callosum was observed by using HE staining,and the expression levels of MBP protein and mRNA in the right corpus callosum were detected by immunohistochemistry,enzyme-linked immunosorbent assay(ELISA),Western blot(WB)and reverse transcription polymerase chain reaction(RT-PCR).Results:Compared with the sham operation group,the grip strength was decreased,the neurological deficit scores were increased,the percentage of cerebral infarction volume was increased,the structure of the corpus callosum myelin was disintegrated,and the expression levels of MBP protein and mRNA in the right corpus callosum were decreased in the model group,with statistically significant differences(P<0.05).Compared with the model group and non-acupoint group,the grip strength was increased,the neurological deficit scores were decreased,the percentage of cerebral infarc

关 键 词：巨刺 阳陵泉穴 电针 脑缺血 胼胝体 髓鞘碱性蛋白 中风康复 大鼠 大脑中动脉栓塞 

分 类 号：R289.1[医药卫生—方剂学]