基于文献挖掘的过敏性紫癜动物模型应用特点分析及建模思考  

Analysis and Modeling Thinking on the Application Characteristics of Animal Models of Henoch-Schönlein Purpura Based on Literature Mining

作　　者：姜盈盈 任献青[1,2,3] 席乐迎 邢琼琼 蔡明阳 杨满翔 李一凡 宋金婉 JIANG Yingying;REN Xianqing;XI Leying;XING Qiongqiong;CAI Mingyang;YANG Manxiang;LI Yifan;SONG Jinwan(Henan University of Chinese Medicine,Zhengzhou 450046,China;College of Pediatrics,Henan University of Chinese Medicine,Zhengzhou 450000,China;The First Affiliated Hospital of Henan University of Chinese Medicine,Zhengzhou 450008,China)

机构地区：[1]河南中医药大学,郑州450046 [2]河南中医药大学儿科医学院,郑州450000 [3]河南中医药大学第一附属医院,郑州450008

出　　处：《中国中医基础医学杂志》2024年第5期799-806,共8页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：国家自然科学基金项目(U2004107);省级科技研发计划联合基金(优势学科培育类)项目(222301420022);河南省首批拔尖人才专项基金项目(2019ZYBJ01);河南省中医学“双一流”创建科学研究专项课题(HSRP-DFCTCM-2023-8-07)。

摘　　要：目的研究过敏性紫癜动物模型的造模特点,为其动物模型的规范化提供计量参考。方法在中国知网、万方、维普、中国生物医学文献数据库、PubMed及Web of Science数据库检索过敏性紫癜动物模型相关文献,归纳实验动物品系、年龄、性别、造模方法、成模周期、阳性对照药、检测指标等,构建数据库并进行规范整理、分析。结果共纳入符合标准的46篇文献,包含动物模型实验研究52例。实验动物多选择6周龄、3~4周龄的SD大鼠或3~4周龄、8周龄的KM小鼠,性别多选择雄性或雌雄各半。模型以病证结合复合模型和模拟IgA肾病模型多见;常用造模方法为尾静脉注射印度墨水+灌胃和尾静脉注射麦胶蛋白溶液、灌胃/口服干姜、胡椒、荜茇+腹腔注射卵白蛋白和弗氏完全佐剂混合液+尾静脉/耳缘静脉和皮内注射卵白蛋白0.9%氯化钠溶液;造模周期以14、6、12周多见。阳性对照药应用最多的是西咪替丁、双嘧达莫、雷公藤多苷片。高频检测指标依次为血清相关生化指标、肾组织病理、24 h尿蛋白、肾组织免疫组化、表观指标、尿常规、皮肤组织病理等。结论目前过敏性紫癜动物模型制备方法多样,病证结合复合模型与中西医临床特点吻合度较高,但缺少统一的造模方法及中医证候评价标准等,还需探索其他相关证型的病证模型,并进一步重复验证、完善。Objective To study the modeling characteristics of Henoch-Schönlein purpura animal models,and to provide metrological reference for the standardization.Methods Search for related literatures on animal models of Henoch-Schönlein purpura in China National Knowledge Infrastructure,Wanfang,VIP,China Biology Medicine disc,PubMed and Web of Science Database,summarize the experimental animal strain,age,sex,modeling method,modeling cycle,positive control drug,detection index,etc.,construct the database and standardize and analyze it.Results A total of 46 articles in accordance with the criteria were included,including 52 animal models experiments.Most of the experimental animals chose 6-and(3-4)-week-old SD rats or(3-4)and 8-week-old KM mice.And the sex was mostly male,or half male and half female.The principle of modeling was the combination of disease and syndrome,and the simulation of IgA nephropathy.The common modeling methods were tail vein injection of Indian ink+intragastric and caudal vein injection of wheat collagen solution,stomach administration/oral administration of dried ginger,pepper,Piper longum+intraperitoneal injection of ovalbumin and Freund's complete adjuvant mixture+caudal vein/auricular vein and intradermal injection of ovalbumin saline solution.The modeling period was 14,6,12 weeks.The most commonly used positive control drugs were cimetidine,dipyridamole and tripterygium wilfordii polyglycosides tablets.The high-frequency detection indexes were serum-related biochemical indexes,renal histopathology,24-hour urinary protein,renal tissue immunohistochemistry,apparent index,urine routine,skin histopathology and so on.Conclusion At present,there are various animal models of Henoch-Schönlein purpura,and the compound model of combination of disease and syndrome is highly consistent with the clinical characteristics of Chinese and western medicine.But there is a lack of unified model preparation method and TCM syndrome evaluation standard.So it is necessary to explore the disease and syndrome models o

关 键 词：过敏性紫癜 动物模型 数据挖掘 病证结合 

分 类 号：R554.6[医药卫生—血液循环系统疾病] R-332[医药卫生—内科学]