负载纳米钽的液晶显示光固化聚乳酸支架制备及促成骨性能  

Preparation and osteogenic properties of liquid crystal display light-cured polylactic acid scaffold loaded with nano-tantalum

作　　者：李明哲[1] 叶翔凌 王冰 余翔[3,4] Li Mingzhe;Ye Xiangling;Wang Bing;Yu Xiang(Department of Acupuncture and Orthopedics,Nanyang Medical College,Nanyang 473000,Henan Province,China;Dongguan Hospital,Guangzhou University of Chinese Medicine,Dongguan 523888,Guangdong Province,China;First Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510405,Guangdong Province,China;Guangdong Clinical Research Institute of Chinese Medicine,Guangzhou 510405,Guangdong Province,China)

机构地区：[1]南阳医学高等专科学校针灸骨伤系,河南省南阳市473000 [2]广州中医药大学东莞医院,广东省东莞市523888 [3]广州中医药大学第一附属医院,广东省广州市510405 [4]广东省中医临床研究院,广东省广州市510405

出　　处：《中国组织工程研究》2025年第4期670-677,共8页Chinese Journal of Tissue Engineering Research

基　　金：河南省高等职业院校创新发展行动计划项目-李氏针灸工作室(XM-17-439),项目负责人:李明哲;国家自然科学基金项目(82305264),项目负责人:余翔;广州市青年科技人才托举项目(QT-2023-022),项目负责人:余翔;市校(院)联合资助(登峰医院)基础研究项目(202201020307),项目负责人:余翔;广州中医药大学第一附属医院中青年骨干人才培育项目-青优人才(2023QY13),项目负责人:余翔。

摘　　要：背景:聚乳酸因具有良好的生物相容性和可控的降解速率在生物医学工程中得到了广泛应用,然而存在机械强度低和生物活性不足等缺陷,限制了其在骨组织工程中的进一步应用。目的:构建聚乳酸/聚多巴胺/钽(PLA/PDA/Ta)骨组织工程支架,探究其生物安全性和体外促成骨性能。方法:利用液晶显示光固化技术制备具有多孔结构的聚乳酸(PLA)支架,将PLA支架分别浸泡在多巴胺溶液与多巴胺-纳米钽混合溶液中分别制备聚乳酸/聚多巴胺(PLA/PDA)支架、PLA/PDA/Ta支架,表征支架的微观形貌与水接触角。将MC3T3-E1细胞分别与PLA、PLA/PDA、PLA/PDA/Ta支架共培养,进行CCK-8检测与活/死细胞染色;成骨诱导分化后,进行碱性磷酸酶、茜素红染色及成骨基因检测。结果与结论:①扫描电镜下可见3种支架均具有互连的多孔三维结构,平均孔径为200μm;PLA/PDA/Ta支架的水接触角低于PLA、PLA/PDA支架(P<0.05);②CCK-8检测显示,相较于PLA、PLA/PDA支架,PLA/PDA/Ta支架可促进细胞的增殖(P<0.05);活/死细胞染色显示3组细胞增殖良好;③碱性磷酸酶与茜素红染色显示,相较于PLA、PLA/PDA支架,PLA/PDA/Ta支架可促进细胞碱性磷酸酶的表达与矿化结节形成;RT-qPCR检测显示,相较于PLA、PLA/PDA支架,PLA/PDA/Ta支架可促进细胞骨形态发生蛋白、Runx-2及Ⅰ型胶原mRNA的表达(P<0.05,P<0.01);④结果表明,PLA/PDA/Ta支架具有优异的促细胞增殖与成骨活性。BACKGROUND:Polylactic acid(PLA)has good biocompatibility and a controllable degradation rate and is currently widely used in biomedical engineering.However,PLA has shortcomings such as low mechanical strength and insufficient biological activity,which limits its further application in bone tissue engineering.OBJECTIVE:To construct polylactic acid/polydopamine/tantalum(PLA/PDA/Ta)bone tissue engineering scaffolds,and explore their biosafety and in vitro osteogenesis.METHODS:A PLA scaffold with a porous structure was prepared through liquid crystal display light-curing technology.PLA/PDA scaffolds and PLA/PDA/Ta scaffolds were prepared by soaking PLA scaffolds in dopamine solution and dopamine-tantalum nanoparticle solution,respectively.The microstructure and water contact angle of scaffolds were characterized.MC3T3-E1 cells were co-cultured with PLA,PLA/PDA,and PLA/PDA/Ta scaffolds,respectively,and CCK-8 assay and live/dead cell staining were performed.After osteogenic differentiation,alkaline phosphatase,alizarin red staining,and osteogenic gene detection were performed.RESULTS AND CONCLUSION:(1)The scanning electron microscope results exhibited that the three kinds of prepared scaffolds had an interconnected porous three-dimensional structure,and the average pore diameter was 200μm.The water contact angle of PLA/PDA/Ta scaffolds was lower than that of PLA and PLA/PDA scaffolds(P<0.05).(2)CCK-8 assay showed that compared with PLA and PLA/PDA scaffolds,PLA/PDA/Ta scaffolds could promote cell proliferation(P<0.05).Live/dead cell staining showed good cell proliferation in the three groups.(3)Alkaline phosphatase and alizarin red staining showed that compared with PLA and PLA/PDA scaffolds,PLA/PDA/Ta scaffolds could promote the expression of alkaline phosphatase and the formation of mineralized nodules.RT-qPCR showed that compared with PLA and PLA/PDA scaffolds,PLA/PDA/Ta scaffolds could enhance the mRNA expression of cell bone morphogenetic protein,Runx-2,and type I collagen(P<0.05,P<0.01).(4)The results showed tha

关 键 词：液晶显示光固化打印 聚乳酸 聚多巴胺 纳米钽 MC3T3-E1细胞 骨组织工程支架 

分 类 号：R459.9[医药卫生—治疗学] R318.6[医药卫生—临床医学] R683