红景天苷促进MC3T3-E1细胞成骨分化能力的体外实验  

Salidroside promotes osteogenic differentiation of MC3T3-E1 cells:an in vitro experiment

作　　者：刘朝辉 韩晓谦[1] 段昕 郭鹏达 张云涛[1] Liu Zhaohui;Han Xiaoqian;Duan Xin;Guo Pengda;Zhang Yuntao(Department of Stomatology,Affiliated Hospital of Binzhou Medical University,Binzhou 256600,Shandong Province,China;Maternal and Child Health Hospital of Linshu County,Linyi 276700,Shandong Province,China)

机构地区：[1]滨州医学院附属医院口腔科,山东省滨州市256600 [2]山东省临沭县妇幼保健院,山东省临沂市276700

出　　处：《中国组织工程研究》2025年第2期231-237,共7页Chinese Journal of Tissue Engineering Research

基　　金：2020山东省专业学位研究生提升计划(SDYAL20202),项目负责人:张云涛。

摘　　要：背景:骨缺损可直接影响牙齿种植的成功率及种植体的长期稳定性。研究显示红景天苷可促进成骨细胞增殖、分化,但对于其成骨分化相关通路具体研究不多。目的:体外细胞实验研究红景天苷对MC3T3-E1细胞增殖和分化的影响,并探究其对相关基因和蛋白表达的影响。方法:采用CCK-8实验和碱性磷酸酶实验筛选红景天苷(0.5,1,5,10,50μmol/L)促进MC3T3-E1细胞增殖和分化的最佳浓度。实验分为4组:对照组、红景天苷组、红景天苷+LY294002组、LY294002组,分别用成骨诱导液、含10μmol/L红景天苷、10μmol/L红景天苷+10μmol/L LY294002、10μmol/L LY294002的成骨诱导液进行培养,观察红景天苷及PI3K/Akt信号通路抑制剂LY294002对成骨相关基因和蛋白表达的影响。结果与结论:①CCK-8实验和碱性磷酸酶实验显示:红景天苷促进MC3T3-E1细胞增殖的作用在10μmol/L时最显著;②与对照组相比,红景天苷可以促进矿化、促进细胞黏附、减少细胞死亡,提高Runx-2、骨钙素、骨桥蛋白的mRNA表达(P<0.01),提高Runx-2和p-Akt蛋白表达(P<0.01);而添加PI3K/Akt信号通路抑制剂LY294002则可逆转以上结果;③结果表明,红景天苷能促进MC3T3-E1细胞矿化,并能促进成骨相关基因、蛋白的表达,这可能与PI3K/Akt信号通路的激活有关。BACKGROUND:Bone defects can directly affect the success rate and long-term stability of dental implants.Studies have shown that salidroside has the ability to promote the proliferation and differentiation of osteoblasts,but less is reported on its pathways related to osteogenic differentiation.OBJECTIVE:To investigate the effects of salidroside on the proliferation and differentiation of MC3T3-E1 cells and the expression of related genes and proteins through in vitro cell experiments.METHODS:Cell counting kit-8 test and alkaline phosphatase test were used to determine the optimal concentration of salidroside(0.5,1,5,10,and 50μmol/L)in promoting the proliferation and differentiation of MC3T3-E1 cells.There were four groups in the experiment:control group,salidroside group,salidroside+LY294002 group,and LY294002 group,which were cultured with osteogenic induction solution,osteogenic induction solution containing 10μmol/L salidroside,osteogenic induction solution containing 10μmol/L salidroside+10μmol/L LY294002,and osteogenic induction solution containing 10μmol/L LY294002,respectively.The effects of salidroside and LY294002,an inhibitor of the PI3K/Akt signaling pathway,on the expressions of genes and proteins related to osteogenesis were observed.RESULTS AND CONCLUSION:Cell counting kit-8 assay and alkaline phosphatase assay showed that salidroside promoted the proliferation of MC3T3-E1 cells most significantly at 10μmol/L.Compared with the control group,salidroside could promote mineralization,promote cell adhesion,reduce cell death,increase mRNA expression of Runx-2,osteocalcin and osteopontin(P<0.01),and increase protein expression of Runx-2 and p-Akt(P<0.01).However,the addition of LY294002 reversed the above results.These findings indicate that salidroside can promote the mineralization of MC3T3-E1 cells and the expression of osteogenesis-related genes and proteins,which may be related to the activation of PI3K/Akt signaling pathway.

关 键 词：红景天苷 MC3T3-E1细胞 细胞分化 成骨 矿化 LY294002 PI3K/AKT信号通路 

分 类 号：R496[医药卫生—康复医学] R318[医药卫生—临床医学] R446