游泳运动对大鼠创伤性关节挛缩的影响及机制  

Effects and mechanisms of swimming for inhibiting traumatic joint contracture in a rat model

作　　者：税晓平[1,2] 李春莹 张鑫[4] 李彬[2] 冯超 周宏宇 陈科 廖莹莹 Shui Xiaoping;Li Chunying;Zhang xin;Li Bin;Feng Chao;Zhou Hongyu;Chen Ke;Liao Yingying(Sichaun College of Traditional Chinese Medicine,Mianyang 621000,Sichuan Province,China;Mianyang Orthopedic Hospital,Mianyang 621000,Sichuan Province,China;Mianyang Hospital of Traditional Chinese Medicine,Mianyang 621000,Sichuan Province,China;Sichuan Province Orthopedic Hospital,Chengdu 610041,Sichuan Province,China)

机构地区：[1]四川中医药高等专科学校,四川省绵阳市621000 [2]绵阳市骨科医院,四川省绵阳市621000 [3]绵阳市中医医院,四川省绵阳市621000 [4]四川省骨科医院,四川省成都市610041

出　　处：《中国组织工程研究》2025年第2期262-268,共7页Chinese Journal of Tissue Engineering Research

基　　金：2023年度中国康复医学会立项科技发展培育项目(培育项目14),项目负责人:税晓平;四川中医药高等专科学校科研创新团队(TD-2022-04),项目负责人:税晓平;绵阳市骨科医院科研创新团队和绵阳市高等教育“三三工程”(优势特色专业-康复治疗技术专业建设)项目(202055),项目负责人:税晓平。

摘　　要：背景:早期运动是预防创伤性关节挛缩的主要方式,也是近期研究热点。游泳运动借助水的特殊物理特性,可能是潜在有益的干预方式。目的:观察游泳运动对大鼠关节挛缩发展的影响,探究游泳运动预防关节挛缩的相关机制。方法:24只SD大鼠随机分为空白对照组(n=8)和关节挛缩造模组(n=16),手术制备膝关节挛缩模型后,再随机分为手术对照组(n=8)和游泳干预组(n=8)。游泳干预组在术后第2周开始游泳干预,共干预5周。在术后第6周,测试各组大鼠体质量、术侧膝关节活动度、股四头肌直径,计算直径-体质量指数;苏木精-伊红染色观察膝关节囊和股四头肌病理变化;Masson染色观察关节囊胶原纤维化改变;免疫组化检测膝关节囊中转化生长因子β1和Ⅰ型胶原蛋白表达;Western blot检测股四头肌中MuRF1蛋白的表达。结果与结论:①与空白对照组比较,手术对照组和游泳干预组大鼠膝关节活动度下降,总伸膝受限角度和关节源性伸膝受限角度均明显增加(P<0.01),股四头肌直径下降(P<0.01),关节囊出现明显纤维化表现,股四头肌萎缩,手术对照组直径-体质量指数降低(P<0.01);与手术对照组比较,游泳干预组大鼠膝关节活动度和股四头肌直径明显增加(P<0.01),关节囊纤维化病变和股四头肌萎缩明显改善;②与空白对照组比较,手术对照组和游泳干预组大鼠关节囊中胶原纤维含量、转化生长因子β1和Ⅰ型胶原蛋白表达均增加(P<0.01);与手术对照组比较,游泳干预组大鼠关节囊中胶原纤维含量、转化生长因子β1和Ⅰ型胶原蛋白表达均降低(P<0.01);③与空白对照组比较,手术对照组和游泳干预组大鼠股四头肌中MuRF1蛋白表达增加(P<0.05);与手术对照组比较,游泳干预组大鼠股四头肌中MuRF1蛋白表达降低(P<0.05)。结果表明:早期游泳干预能够降低创伤性挛缩大鼠关节囊中转化生长因子β1和Ⅰ型胶原蛋白表�BACKGROUND:Early exercise treatment is the main prevention way for traumatic joint contracture and is also a research focus.Swimming may be a potential intervention for joint contracture due to the special physical properties of water.OBJECTIVE:To explore the effects of swimming on the development of joint contracture in a rat model and study its mechanisms.METHODS:Twenty-four Sprague-Dawley rats were randomly divided into a blank control group(n=8)and a joint contracture group(n=16).After the surgical operation of knee joint contracture rat models,the joint contracture group was randomly subdivided into a surgical control group(n=8)and a swimming treatment group(n=8).Swimming started in the swimming treatment group in the second week after surgery and lasted for a total of 5 weeks.At the 6th week after surgery,the body mass,knee joint range of motion,and quadriceps diameter were tested,and the diameter/body mass index was calculated.Hematoxylin-eosin staining was performed to detect the pathological changes in the knee joint capsule and quadriceps muscle,and Masson staining was used to observe fibrotic changes in the knee joint capsule.Furthermore,the protein expression of transforming growth factorβ1 and type I collagen in the knee joint capsule was quantified by immunohistochemical assay and western blot was performed to detect the protein expression of MuRF1 in the quadriceps femoris.RESULTS AND CONCLUSION:Compared with the blank control group,the knee range of motion decreased in the surgical control and swimming treatment groups(P<0.01),and knee extension deficit and arthrogenic extension deficit were significantly increased(P<0.01),the diameter of the quadriceps muscle was decreased(P<0.01),the joint capsule showed significant fibrosis,the quadriceps muscle was atrophied,and the diameter/body mass index was decreased(P<0.01).Compared with the surgical control group,the swimming treatment group showed a significant increase in knee joint range of motion and quadriceps diameter(P<0.01),and significant impro

关 键 词：关节挛缩 肌肉萎缩 游泳 运动 关节活动度 大鼠 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学] R684