不同强度运动干预2型糖尿病大鼠骨骼肌羧酸酯酶1及炎症因子的变化  

Effects of different exercise interventions on carboxylesterase 1 and inflammatory factors in skeletal muscle of type 2 diabetic rats

作　　者：胡淑娟 程平 张啸 丁一庭 刘璇[1] 蒲锐 汪献旺 Hu Shujuan;Cheng Ping;Zhang Xiao;Ding Yiting;Liu Xuan;Pu Rui;Wang Xianwang(School of Education and Physical Education,Yangtze University,Jingzhou 423000,Hubei Province,China;College of Sports Science,Jishou University,Jishou 416000,Hunan Province,China;Department of Medicine,Yangtze University,Jingzhou 423000,Hubei Province,China)

机构地区：[1]长江大学教育与体育学院,湖北省荆州市423000 [2]吉首大学体育科学学院,湖南省吉首市416000 [3]长江大学医学部,湖北省荆州市423000

出　　处：《中国组织工程研究》2025年第2期269-278,共10页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目(81974115);湖北省重点科技研发计划项目(2021BGD010),项目负责人:汪献旺;湖北省教育厅科研计划项目(D20201306),项目负责人:汪献旺;长江大学大学生创新创业训练计划项目(Yz2022307,Yz2022164,Yz2023165);长江大学社会科学基金重点项目(2022csz07),项目负责人:胡淑娟。

摘　　要：背景:羧酸酯酶1及炎症因子在调节脂质代谢以及葡萄糖稳态方面起着至关重要的作用,然而有关不同强度运动干预对2型糖尿病大鼠骨骼肌羧酸酯酶1及炎症因子的影响尚待揭示。目的:探究不同强度运动干预对2型糖尿病大鼠骨骼肌羧酸酯酶1及炎症因子的影响。方法:取32只8周龄雄性SD大鼠,适应性喂养1周后随机分为正常对照组(n=12)和造模组(n=20),造模组大鼠利用高脂膳食和一次性注射链脲佐菌素制备2型糖尿病模型,建模成功后随机分为糖尿病对照组(n=6)、中等强度运动组(n=6)和高强度间歇运动组(n=6),后2组适应性跑台运动5 d后分别进行对应强度的跑台运动,每天1次,每次50 min,每周训练5 d。连续运动6周后,检测大鼠血糖与血脂相关指标,苏木精-伊红染色观察骨骼肌组织形态学变化,qRT-PCR检测骨骼肌羧酸酯酶1与炎症因子的mRNA表达,Western-blotting及免疫荧光染色检测骨骼肌中羧酸酯酶1与炎症因子的蛋白表达。结果与结论:①与正常对照组相比,糖尿病对照组大鼠空腹血糖、三酰甘油、低密度脂蛋白胆固醇、胰岛素抵抗指数均升高(P<0.05),胰岛素活性降低(P<0.05),骨骼肌中的羧酸酯酶1、NEK7、白细胞介素18的mRNA与蛋白表达均升高(P<0.05)。②与糖尿病对照组相比,中等强度运动组和高强度间歇运动组大鼠空腹血糖、三酰甘油、低密度脂蛋白胆固醇、胰岛素抵抗指数均降低(P<0.05),胰岛素活性升高(P<0.05);中等强度运动组大鼠骨骼肌中的NEK7 mRNA表达降低(P<0.01),羧酸酯酶1、NEK7、白细胞介素18蛋白表达降低(P<0.05);高强度间歇运动组大鼠骨骼肌中的羧酸酯酶1、NEK7、NLRP3、白细胞介素18 mRNA表达降低(P<0.05),羧酸酯酶1、白细胞介素18蛋白表达降低(P<0.05)。③苏木精-伊红染色显示,相较于糖尿病对照组,中等强度运动组大鼠肌纤维间隙变小,内部空洞减少,细胞结构趋于完整;高强度间�BACKGROUND:Carboxylesterase 1 and inflammatory factors play a crucial role in regulating lipid metabolism and glucose homeostasis.However,the effects of different exercise intensity interventions on carboxylesterase 1 and inflammatory factors in skeletal muscle of type 2 diabetic rats remain to be revealed.OBJECTIVE:To investigate the effects of different exercise intensity interventions on carboxylesterase 1 and inflammatory factors in skeletal muscle of type 2 diabetic rats.METHODS:Thirty-two 8-week-old male Sprague-Dawley rats were randomly divided into normal control group(n=12)and modeling group(n=20)after 1 week of adaptive feeding.Rat models of type 2 diabetes mellitus were prepared by high-fat diet and single injection of streptozotocin.After successful modeling,the rats were randomly divided into diabetic control group(n=6),moderate-intensity exercise group(n=6)and high-intensity intermittent exercise group(n=6).The latter two groups were subjected to treadmill training at corresponding intensities,once a day,50 minutes each,and 5 days per week.Exercise intervention in each group was carried out for 6 weeks.After the intervention,ELISA was used to detect blood glucose and blood lipids of rats.The morphological changes of skeletal muscle were observed by hematoxylin-eosin staining.The mRNA expression levels of carboxylesterase 1 and inflammatory cytokines were detected by real-time quantitative PCR.The protein expression levels of carboxylesterase 1 and inflammatory cytokines were detected by western blot and immunofluorescence.RESULTS AND CONCLUSION:Compared with the normal control group,fasting blood glucose,triglyceride,low-density lipoprotein cholesterol,insulin resistance index in the diabetic control group were significantly increased(P<0.01),insulin activity was decreased(P<0.05),and the mRNA and protein levels of carboxylesterase 1,never in mitosis gene A related kinase 7(NEK7)and interleukin 18 in skeletal muscle tissue were upregulated(P<0.05).Compared with the diabetic control group,fasting blo

关 键 词：中等强度有氧运动 高强度间歇运动 2型糖尿病 羧酸酯酶1 炎症因子 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学] R587.1