高强度间歇性运动干预2型糖尿病患者糖代谢及肠道菌群的变化  

Changes in glucose metabolism and intestinal flora in patients with type 2 diabetes mellitus after high-intensity intermittent exercise

作　　者：余杭林 田浩冬 文世媛 黄丽 刘昊为 李汉森 王培松 彭莉[1] Yu Hanglin;Tian Haodong;Wen Shiyuan;Huang Li;Liu Haowei;Li Hansen;Wang Peisong;Peng Li(School of Physical Education and Sports,Southwest University,Chongqing 400700,China;Department of Critical Care Medicine,The First People’s Hospital of Yunnan Province,Affiliated Hospital of Kunming University of Science and Technology,Kunming 650000,Yunan Province,China;Shenzhen Pingshan District Foreign Language Source School,Shenzhen 518000,Guangdong Province,China)

机构地区：[1]西南大学体育学院,重庆市400700 [2]云南省第一人民医院,昆明理工大学附属医院重症医学科,云南省昆明市650000 [3]深圳市坪山区外国语文源学校,广东省深圳市518000

出　　处：《中国组织工程研究》2025年第2期286-293,共8页Chinese Journal of Tissue Engineering Research

基　　金：国家社会科学基金(21BTY092),项目名称:慢性病人群健康行为促进的社区体医融合模式研究,项目负责人:彭莉;重庆市自然科学基金项目(cstc2020jcyj-msxmX1025),项目名称:运动干预Ⅱ型糖尿病的肠道菌群机制研究,项目负责人:彭莉。

摘　　要：背景:运动对肠道菌群和糖代谢具有调节作用,但高强度间歇性运动对2型糖尿病患者肠道菌群和糖代谢的影响尚不明确。目的:探究高强度间歇性运动对2型糖尿病患者糖代谢及肠道菌群的影响。方法:招募11例2型糖尿病患者,脱落2例,最终有9例进入试验。对患者进行为期6周、每周3次的高强度间歇性运动干预,干预前后收集受试者的空腹血液和粪便样本,对血液样本进行糖代谢指标检测,对粪便样本进行肠道菌群检测,比较干预前后2型糖尿病患者糖代谢指标和肠道菌群指标的变化。结果与结论:①患者经6周的高强度间歇性运动干预后,血液指标中的空腹血糖和糖化血清蛋白水平显著降低(P<0.05);空腹胰岛素虽无显著变化,但比干预前有所下降;②Alpha多样性分析显示,患者肠道菌群群落的多样性(Shannon指数)、丰富度(Chao指数)和覆盖度(Coverage指数)无显著变化;③Venn图显示,患者肠道菌群中的拟杆菌门、放线菌门、变形菌门和梭杆菌门的相对丰度增加,厚壁菌门的相对丰度降低,显著降低的是厚壁菌门中的扭链瘤胃球菌与活泼瘤胃球菌,这两种菌属均与糖代谢相关性指标异常以及其他疾病的发展呈正相关;④结果显示,高强度间歇性运动干预对2型糖尿病患者糖代谢相关指标具有改善作用,患者肠道中的有益菌群丰度增加,有害菌群丰度减少,增强了患者肠道菌群的稳定性。BACKGROUND:Exercise has a regulatory effect on intestinal flora and glucose metabolism,but the effects of high-intensity intermittent exercise on intestinal flora and glucose metabolism in patients with type 2 diabetes mellitus are unclear.OBJECTIVE:To investigate the effects of high-intensity intermittent exercise on glucose metabolism and intestinal flora in patients with type 2 diabetes mellitus.METHODS:Eleven patients with type 2 diabetes mellitus were recruited,among which,two were lost to the follow-up and nine were finally enrolled.Highintensity intermittent exercise intervention was conducted 3 times per week for 6 continuous weeks.Fasting blood and fecal samples were collected before and after the intervention.Glucose metabolism indexes were detected in the blood samples,and intestinal flora was detected in the fecal samples.Changes in glucose metabolism indexes and intestinal flora indexes of the patients with type 2 diabetes mellitus before and after the intervention were compared.RESULTS AND CONCLUSION:After 6 weeks of high-intensity intermittent exercise intervention,fasting blood glucose and glycosylated serum protein levels in patients were significantly reduced(P<0.05),and fasting insulin,although not significantly changed,was decreased compared with before intervention.Alpha diversity analysis showed that the diversity(Shannon index),richness(Chao index)and coverage(Coverage index)did not change significantly.Venn diagrams showed that the relative abundance of Bacteroidetes,Actinobacteria,Proteobacteria,and Fusobacteria in the intestinal flora of the patients increased,and the relative abundance of Firmicutes decreased,and a significant decrease was seen in Ruminococcus_torques and Ruminococcus_gnavus in the Firmicutes,which were both positively correlated with the abnormalities of the glycemic metabolism-related indicators,as well as with other disease development.All these findings indicate that high-intensity intermittent exercise intervention has an improvement effect on the glycemic metaboli

关 键 词：高强度间歇性运动 2型糖尿病 肠道菌群 糖代谢 干预 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学] R587.1