改良结扎法用于脊髓损伤造模的优势分析  

Advantages of modified ligation method for spinal cord injury modeling

作　　者：李道辉 徐晓霜 李郑涛 田新鹏 毕航川 刘源[1] 戴永文 陈凌强[1] Li Daohui;Xu Xiaoshuang;Li Zhengtao;Tian Xinpeng;Bi Hangchuan;Liu Yuan;Dai Yongwen;Chen Lingqiang(Department of Orthopaedics,The First Affiliated Hospital of Kunming Medical University,Kunming 650032,Yunnan Province,China;School of Public Health,Kunming Medical University,Kunming 650500,Yunnan Province,China;Xichang People’s Hospital,Liangshan Yi Autonomous Prefecture 615000,Sichuan Province,China)

机构地区：[1]昆明医科大学第一附属医院骨科,云南省昆明市650032 [2]昆明医科大学公共卫生学院,云南省昆明市650500 [3]西昌市人民医院,四川省凉山彝族自治州615000

出　　处：《中国组织工程研究》2025年第2期379-384,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目(81660215,81860093),项目负责人:陈凌强;云南省科技厅科技计划项目(202101AT070226,202101AY070001-31),项目负责人:陈凌强。

摘　　要：背景:目前根据脊髓损伤的损伤方式不同衍生出不同的模型构建方法,传统物理损伤造模方法都有各自的优缺点,尚缺乏较为有效和稳定的脊髓损伤动物模型。目的:建立一种可复制、可调控、创伤小、死亡率低、模型更稳定、适用范围广、术后护理时间短的脊髓损伤大鼠模型。方法:将体质量和鼠龄相似的40只SD大鼠随机分为对照组和改良组,每组20只,对照组采用钳夹造模法构建脊髓损伤模型,改良组选用基于压迫法改良的结扎法,在开窗的基础上使用缝线结扎的方法进行脊髓损伤的造模。比较两组大鼠的排尿行为、血尿、脓尿(感染率)、死亡率、脊柱畸形率及术后1,3,5,7 d的BBB运动功能学评分。结果与结论:基于压迫法改良的结扎法建立的脊髓损伤大鼠模型比常规建模方法排尿行为恢复较快、血尿率低、感染率低、死亡率低、脊柱畸形率低,BBB评分结果更集中稳定(1周内均<2分),由此可证明基于压迫法改良的结扎法更适用于脊髓损伤大鼠模型的建立。BACKGROUND:Currently,different methods of model establishment have been derived from different injury modes of spinal cord injury.Traditional physical injury modeling methods have their own advantages and disadvantages,and there is a lack of more effective and stable animal models of spinal cord injury.OBJECTIVE:To establish a reproducible,controllable,trauma-free,low-mortality,more stable,widely applicable,and short-term postoperative care rat model of spinal cord injury.METHODS:Forty Sprague-Dawley rats with similar body mass and ages were randomly divided into a control group and an improved group,with 20 rats in each group.Animal models of spinal cord injury in the control group were constructed using a clip model method,while the improved group used a modified ligation method based on the compression method to make the spinal cord injury models using suture ligation based on fenestration.Postoperative comparisons were made between the two groups,assessing urination behavior,hematuria,pyuria(infection rate),mortality,scoliosis rate and Basso-Beattie-Bresnahan locomotor rating scale scores at 1,3,5,and 7 days after modeling.RESULTS AND CONCLUSION:Compared with the conventional modeling method,the modified ligation method based on the compression method resulted in faster recovery of urination behavior,lower hematuria rate,lower infection rate,lower mortality rate,lower scoliosis rate,and more concentrated and stable Basso-Beattie-Bresnahan scores(all below 2 points within 1 week).This proves that the modified ligation method based on compression is more suitable for the establishment of spinal cord injury models in rats.

关 键 词：改良 脊髓损伤模型 钳夹造模法 结扎法 排尿反射 排尿行为 脊柱畸形 BBB评分 

分 类 号：R496[医药卫生—康复医学] R318[医药卫生—临床医学] R446