尿中多环芳烃羟基代谢物浓度与睡眠质量的关联性研究  

Association of polycyclic aromatic hydrocarbon metabolite concentration with sleep quality in workers

作　　者：任琴 李新 张志艳 王馨 宋展飞 张红梅 REN Qin;LI Xin;ZHANG Zhiyan;WANG Xin;SONG Zhanfei;ZHANG Hongmei(Department of Environmental Health,School of Public Health,Shanxi Medical University,Taiyuan,Shanxi 030001,China;Department of Radiological Health,General Hospital of Iron and Steel Company Limited,Taiyuan,Shanxi 030003,China)

机构地区：[1]山西医科大学公共卫生学院环境卫生学教研室,山西太原030001 [2]太原钢铁有限公司总医院放射卫生科,山西太原030003

出　　处：《环境与职业医学》2024年第3期303-310,共8页Journal of Environmental and Occupational Medicine

基　　金：国家自然科学基金项目(81973093);山西省留学人员科技活动择优资助项目(2019028);细胞生理学教育部重点实验室(山西医科大学)开放基金重点项目(KLMEC/SXMU-202004)。

摘　　要：[背景]睡眠是人体必需的生理活动,研究发现睡眠质量受空气污染的影响。多环芳烃(PAHs)作为空气污染物中的神经毒性化合物,其与睡眠质量的关联尚不明确。[目的]探讨PAHs暴露与睡眠质量之间的关联性,为改善睡眠质量提供基础资料。本研究采用横断面调查,以职业性接触PAHs的焦化厂工人(632名)为接触组,以该企业能源动力厂工人(477名)为对照组,所有工人均为三班倒轮班制。问卷调查收集其性别、工龄、年龄、教育程度、吸烟情况、饮酒情况、食用油炸食品情况、做饭次数、烹饪燃料等基本信息;采集夜班后晨起中段尿,用气相色谱-质谱联用法(GC-MS)检测尿中8种PAHs代谢物(OH-PAHs)浓度;用匹兹堡睡眠质量指数量表(PSQI)评估工人的睡眠质量,PSQI得分越高,表示睡眠质量越差。采用多元线性回归模型、贝叶斯核机回归(BKMR)、分位数g分析工人尿中OH-PAHs水平与睡眠质量之间的关联性。[结果]接触组OH-PAHs浓度的中位数及第25、75百分位数[M(P_(25),P_(75))为88.84(46.27,151.96)μg·L^(-1)],高于对照组的浓度[54.33(24.86,97.97)μg·L^(-1)];接触组PSQI总分(x±s)(5.16±3.84)高于对照组(4.60±3.17)。多元线性回归分析发现:经自然对数转换后的8种OH-PAHs浓度总和(lnΣ_(8)OH-PAHs)每上升一个单位,PSQI总分升高0.3646(95%CI:0.1337~0.5955);lnΣOH-PAHs_(低环)每上升一个单位,PSQI总分升高0.2954(95%CI:0.0941~0.4967)。BKMR分析表明:当lnΣ_(8)OH-PAHs浓度增加时,PSQI总分逐渐上升;分位数g显示lnΣ_(8)OH-PAHs浓度每上升一个四分位数,PSQI总分增加0.4062%(95%CI:0.1176%~0.6949%)。[结论]接触组工人尿OH-PAHs浓度较高,睡眠质量差,OH-PAHs浓度与工人睡眠质量呈负相关。[Background]Sleep is a crucial physiological activity for the human body,and research has shown that air pollution can affect sleep quality.However,the association between polycyclic aromatic hydrocarbons(PAHs)exposure,neurotoxic compounds in air pollutants,and sleep quality remains uncertain.[Objective]To evaluate the association of PAHs exposure with sleep quality,and to provide evidence for improving sleep quality.[Methods]This study used a cross-sectional design.We selected 632 workers from a coking plant of a large state-owned enterprise as the exposure group,and 477 workers from the energy and power plant of the same enterprise as the control group.All workers worked in three shifts.A questionnaire survey was conducted to collect basic information including gender,years of service,age,educational level,smoking,alcohol consumption,consumption of fried foods,cooking frequency,types of cooking fuels.Worker's post-shift morning midstream urine was sampled to determine the concentrations of eight PAHs metabolites(OH-PAHs)using gas chromatography-tandem mass spectrometry(GC-MS).Worker's sleep quality was assessed using Pittsburgh Sleep Quality Index(PSQI).A higher PSQI score indicated a lower sleep quality.Associations of urinary OH-PAHs levels with sleep quality in the workers were analyzed using linear regression,Bayesian kernel-machine regression(BKMR),and quantile g-computation.[Results]The median(P_(25),P_(75))concentration of total OH-PAHs in the exposure group[88.84(46.27,151.96)μg·L^(-1)]was higher than that in the control group[54.33(24.86,97.97)μg·L^(-1)].Additionally,the PSQI score(x±s)in the exposure group(5.16±3.84)was higher than that in the control group(4.60±3.17).The multiple linear regression revealed that an increase in the sum of the concentrations of eight OH-PAHs after natural logarithmic transformation(lnΣ_(8)OH-PAHs)was associated with an increase of 0.3646(95%CI:0.1337,0.5955)in PSQI score,and an increase in lnΣlow-ring OH-PAHs was associated with an increase of 0.2954(95%CI:0.09

关 键 词：多环芳烃 羟基代谢物 睡眠质量 贝叶斯核机回归 分位数g 

分 类 号：R135[医药卫生—劳动卫生]