In Vivo Voltammetric Imaging of Metal Nanoparticle-Catalyzed Single-Cell Electron Transfer by Fermi Level-Responsive Graphene  

作　　者：Qing Xia Rui Liu Xueqin Chen Zixuan Chen Jun-Jie Zhu 

机构地区：[1]State Key Laboratory of Analytical Chemistry for Life Science,School of Chemistry and Chemical Engineering,Nanjing University,Nanjing 210023,P.R.China [2]Shenzhen Research Institute of Nanjing University,Shenzhen 518000,P.R.China

出　　处：《Research》2024年第1期271-280,共10页研究（英文）

基　　金：the National Natural Science Foundation of China(grants nos.22122405,22174061,and 21974065);the Natural Science Foundation of Jiangsu Province(grant no.BK20200059);the Guangdong Basic and Applied Basic Research Foundation(grant no.2020B1515120026);the Funds for Central Guided Regional Science and Technology Development(grant no.2021Szvup058);the Fundamental Research Funds for the Central Universities(grant no.020514380307).

摘　　要：Metal nanomaterials can facilitate microbial extracellular electron transfer(EET)in the electrochemically active biofilm.However,the role of nanomaterials/bacteria interaction in this process is still unclear.Here,we reported the single-cell voltammetric imaging of Shewanella oneidensis MR-1 at the single-cell level to elucidate the metal-enhanced EET mechanism in vivo by the Fermi level-responsive graphene electrode.Quantified oxidation currents of~20 fA were observed from single native cells and gold nanoparticle(AuNP)-coated cells in linear sweep voltammetry analysis.On the contrary,the oxidation potential was reduced by up to 100 mV after AuNP modification.It revealed the mechanism of AuNP-catalyzed direct EET decreasing the oxidation barrier between the outer membrane cytochromes and the electrode.Our method offered a promising strategy to understand the nanomaterials/bacteria interaction and guide the rational construction of EET-related microbial fuel cells.

关 键 词：FERMI VOLTAMMETRY Electron 

分 类 号：O64[理学—物理化学]