A Novel Poly(3-hexylthiophene) Engineered Interface for Electrochemical Monitoring of Ascorbic Acid During the Occurrence of Glutamate-Induced Brain Cytotoxic Edemas  

作　　者：Zexuan Meng Yuchan Zhang Lu Yang Shuang Zhao Qiang Zhou Jiajia Chen Jiuxi Sui Jian Wang Lizhong Guo Luyue Chang Jialing He Guixue Wang Guangchao Zang 

机构地区：[1]Institute of Life Science,and Laboratory of Tissue and Cell Biology,Lab Teaching and Management Center,Chongqing Medical University,Chongqing 400016,China [2]Key Laboratory for Biorheological Science and Technology of Ministry of Education,State and Local Joint Engineering Laboratory for Vascular Implants,Bioengineering College of Chongqing University,Chongqing 400030,China [3]Jinfeng Laboratory,Chongqing 401329,China [4]Department of Pathophysiology,Chongqing Medical University,Chongqing,China

出　　处：《Research》2024年第1期301-313,共13页研究（英文）

基　　金：the Natural Science Foundation of Chongqing(cstc2020jcyj-msxmX0330);the JinFeng Laboratory Foundation,Chongqing,China(jfkyjf202203001);the Project of Tutorial System of Medical Undergraduate in Lab Teaching and Management Center in Chongqing Medical University(LTMCMTS202109 and LTMCMTS202106);the Project of Scientific Research and Innovative Experiment for College Student in Chongqing Medical University(SRIEP202011,SRIEP202047,and SPIEP202167);the National Project of University Students Innovation and Entrepreneurship Training Program(S202010631016);Chongqing Municipal Education Commission Foundation(CYS22333).

摘　　要：Although neuroelectrochemical sensing technology offers unique benefits for neuroscience research,its application is limited by substantial interference in complex brain environments while ensuring biosafety requirements.In this study,we introduced poly(3-hexylthiophene)(P3HT)and nitrogen-doped multiwalled carbon nanotubes(N-MWCNTs)to construct a composite membrane-modified carbon fiber microelectrode(CFME/P3HT-N-MWCNTs)for ascorbic acid(AA)detection.The microelectrode presented good linearity,selectivity,stability,antifouling,and biocompatibility and exhibited great performance for application in neuroelectrochemical sensing.Subsequently,we applied CFME/P3HT-N-MWCNTs to monitor AA release from in vitro nerve cells,ex vivo brain slices,and in vivo living rat brains and determined that glutamate can induce cell edema and AA release.We also found that glutamate activated the N-methyl-d-aspartic acid receptor,which enhanced Na^(+) and Cl^(−) inflow to induce osmotic stress,resulting in cytotoxic edema and ultimately AA release.This study is the first to observe the process of glutamate-induced brain cytotoxic edema with AA release and to reveal the mechanism.Our work can benefit the application of P3HT in in vivo implant microelectrode construction to monitor neurochemicals,understand the molecular basis of nervous system diseases,and discover certain biomarkers of brain diseases.

关 键 词：RELEASE MICROELECTRODE Engine 

分 类 号：X83[环境科学与工程—环境工程]