Cardiac-Adaptive Conductive Hydrogel Patch Enabling Construction of Mechanical–Electrical Anisotropic Microenvironment for Heart Repair  

作　　者：Xiaoping Song Jifeng Zhang Si Shen Dan Liu Jie Zhang Wenming Yin Genlan Ye Leyu Wang Liu Cai Honghao Hou Xiaozhong Qiu 

机构地区：[1]Central Laboratory,The Fifth Affiliated Hospital,Southern Medical University,Guangzhou,Guangdong 510910,China [2]Department of Anatomy,Neuroscience Laboratory for Cognitive and Developmental Disorders,Medical College of Jinan University,Guangzhou 510630,China [3]Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering,School of Basic Medical Science,Biomaterials Research Center,School of Biomedical Engineering,Southern Medical University,Guangzhou,Guangdong 510515,China

出　　处：《Research》2024年第1期489-507,共19页研究（英文）

基　　金：the National Natural Science Foundation of China(U21A20173,52003113,31922043,82102228,and 32071363);Guangdong Basic and Applied Basic Research Foundation(2021A1515010745 and 2020A1515110356);Medical Research Project of Guangdong Province(A2021462);Science and Technology Planning Project of Guangdong Province(2020B1212060037);Key Research&Development Program of Guangzhou Regenerative Medicine and Health Guangdong Laboratory(2018GZR110104002);Shenzhen Fundamental Research Key Project(JCYJ20200109150641992).

摘　　要：The biomimetic construction of a microstructural–mechanical–electrical anisotropic microenvironment adaptive to the native cardiac tissue is essential to repair myocardial infarction(MI).Inspired by the 3D anisotropic characteristic of the natural fish swim bladder(FSB),a novel flexible,anisotropic,and conductive hydrogel was developed for tissue-specific adaptation to the anisotropic structural,conductive,and mechanical features of the native cardiac extracellular matrix.The results revealed that the originally stiff,homogeneous FSB film was tailored to a highly flexible anisotropic hydrogel,enabling its potential as a functional engineered cardiac patch(ECP).In vitro and in vivo experiments demonstrated the enhanced electrophysiological activity,maturation,elongation,and orientation of cardiomyocytes(CMs),and marked MI repair performance with reduced CM apoptosis and myocardial fibrosis,thereby promoting cell retention,myogenesis,and vascularization,as well as improving electrical integration.Our findings offer a potential strategy for functional ECP and provides a novel strategy to bionically simulate the complex cardiac repair environment.

关 键 词：BLADDER thereby PATCH 

分 类 号：R541[医药卫生—心血管疾病] TQ427.26[医药卫生—内科学]