西瓜种子进行细菌性果斑病检测的研究  

Study on detection of Acidovorax citrulli in watermelon seeds

作　　者：洪铭 张志祥 方小雪 许展豪 朱恩泽 HONG Ming;ZHANG Zhixiang;FANG Xiaoxue;XU Zhanhao;ZHU Enze(Ningbo Huizhen Academy,Ningbo 315016,Zhejiang;Ningbo Weimeng Seed Industry Co.,Ltd.,Ningbo 315016,Zhejiang)

机构地区：[1]宁波市惠贞书院,浙江宁波315016 [2]宁波市微萌种业有限公司,浙江宁波315016

出　　处：《浙江农业科学》2024年第5期1198-1202,共5页Journal of Zhejiang Agricultural Sciences

摘　　要：细菌性果斑病(Acidovorax citrulli)是一种由燕麦嗜酸菌西瓜亚种(Acidovorax avenae subsp.Citrulli)引起的严重危害葫芦科作物的世界性病害,该病主要通过种子远距离传播,如果能够在种植瓜果之前就检测出种子是否带病,就能有效防止果斑病及其他更多的病菌对果农造成经济损失。采用酶联免疫吸附测定法(ELISA)和PCR检测法对5份西瓜种子进行细菌性果斑病检测,旨在对两种不同的检测方法进行比较并探究各自的优劣。经过实验对比两种方法发现,ELISA检测方法实验结果容易受外界影响,对操作要求比较高,且精度不如PCR检测法,但在PCR检测法中引物选择十分重要,引物选择直接关系到实验的准确性,因此对PCR检测细菌性果斑病引物选择方面的探索还要继续进行。Bacterial fruit blotch(Acidovorax citrulli)is a worldwide disease caused by Acidovorax avenae subsp.citrulli,which seriously endangers the crops of the gourd family.The disease is mainly transmitted through seeds at a long distance.If the seeds can be detected before planting,fruit blotch and other pathogens can effectively prevent economic losses to fruit farmers.In this experiment,enzyme-linked immunosorbent assay(ELISA)and PCR detection methods were used to detect bacterial fruit spot disease in 5 watermelon seeds,aiming to compare two different detection methods and explore their respective advantages and disadvantages.After comparing the two methods,it was found that the experimental results of ELISA detection method were easy to be affected by external factors,and the operation requirements were relatively high,and the accuracy was not as good as that of PCR detection method.However,in the PCR detection method,primer selection was very important,and the primer selection was directly related to the accuracy of the experiment.Therefore,the exploration of primer selection for PCR detection of bacterial fruit spot disease should be continued.

关 键 词：细菌性果斑病 西瓜种子 酶联免疫吸附测定法 PCR检测法 

分 类 号：S651[农业科学—果树学]