磁性纳米四氧化三铁对细粒棘球蚴感染小鼠的影响  

Effect of magnetic nano⁃Fe_(3)O_(4) particles on Echinococcus granulosus⁃infected mice

作　　者：李志强[1] 颜明智 刘辉 陈蓓[3] 吕国栋 赵军[3] LI Zhiqiang;YAN Mingzhi;LIU Hui;CHEN Bei;LÜGuodong;ZHAO Jun(Laboratory Animal Center of Xinjiang Medical University,Urumqi,Xingjiang 830011,China;State Key Laboratory of Pathogenesis,Prevention and Treatment of Diseases Highly Prevalent in Central Asia,Clinical Medicine Research Institute,the First Affiliated Hospital of Xinjiang Medical University,Urumqi,Xingjiang 830011,China;Department of Pharmacy,the First Affiliated Hospital of Xinjiang Medical University,Urumqi,Xingjiang 830011,China)

机构地区：[1]新疆医科大学动物实验中心,新疆乌鲁木齐830011 [2]新疆医科大学第一附属医院临床医学研究院,省部共建中亚高发病成因与防治国家重点实验室,新疆乌鲁木齐830011 [3]新疆医科大学第一附属医院药学部,新疆乌鲁木齐830011

出　　处：《热带医学杂志》2024年第2期167-170,289,F0002,共6页Journal of Tropical Medicine

基　　金：国家自然科学基金(82160700);省部共建中亚高发病成因与防治国家重点实验室开放课题项目(SKL-HIDCA-2022-35)。

摘　　要：目的探究磁性纳米四氧化三铁(MNPs-Fe_(3)O_(4))对细粒棘球蚴感染小鼠的影响。方法选取18只细粒棘球蚴感染BALB/C小鼠,随机分为2组,每组9只,分为对照组和MNPs-Fe_(3)O_(4)组。对照组小鼠尾静脉注射生理盐水,MNPs-Fe_(3)O_(4)组小鼠尾静脉注射10 mg/kg MNPs-Fe_(3)O_(4),每周连续给药5 d,给药5周后,对小鼠麻醉解剖,收集小鼠病灶和组织,分析比较对照组与MNPs-Fe_(3)O_(4)组小鼠囊湿重和囊泡数量,对小鼠肝、肾、脑、脾和肺组织进行苏木精-伊红(HE)染色,观察病理结构变化。结果MNPs-Fe_(3)O_(4)粒径在150~160 nm。对照组与MNPs-Fe_(3)O_(4)组小鼠囊湿重和囊泡数量差异均无统计学意义(t=-0.011、-0.406,P均>0.05)。HE染色结果显示MNPs-Fe_(3)O_(4)以磁微粒的形式在肝、肺、脾和肾等脏器产生蓄积,但无炎症反应。结论MNPs-Fe_(3)O_(4)对细粒棘球蚴感染小鼠无治疗作用,但可作为一种潜在的囊型棘球蚴病(CE)治疗药物载体,本研究为MNPs-Fe_(3)O_(4)负载CE治疗药物研发提供了理论基础。Objective To investigate the effect of magnetic nano⁃Fe_(3)O_(4) particles(MNPs⁃Fe_(3)O_(4))on Echinococcus granulosus⁃infected mice.Methods A total of 18 E.granulosus⁃infected BALB/C mice were selected and randomly divided into 2 groups of 9 mice each,into control and MNPs⁃Fe_(3)O_(4) groups.The mice in the control group were injected with saline in the tail vein,and the mice in the MNPs⁃Fe_(3)O_(4) group were injected with 10 mg/kg MNPs⁃Fe_(3)O_(4) in the tail vein,and the mice were administered continuously for 5 days per week.After 5 weeks of administration,the mice were anesthetized and dissected,and the lesions and tissues were collected to analyze and compare the wet weight of the cysts and the number of cysts in the control group and the MNPs⁃Fe_(3)O_(4) group,and the liver,kidney,brain,spleen and lung tissues were stained with hematoxylin⁃eosin(HE)to observe the pathological structural changes.Results The particle size of MNPs⁃Fe_(3)O_(4) was 150-160 nm.The differences in cyst wet weight and cyst number between control group and MNPs⁃Fe_(3)O_(4) group mice were not statistically significant(t=-0.011,-0.406;all P>0.05).HE staining showed that MNPs⁃Fe_(3)O_(4) produced accumulation in the form of magnetic particles in the organs of liver,lung,spleen and kidney,but there was no inflammatory reaction.Conclusion MNPs⁃Fe_(3)O_(4) had no therapeutic effect on E.granulosus⁃infected mice,but could be used as a potential cystic echinococcosis(CE)therapeutic drug carrier,and this study provides a theoretical basis for the development of MNPs⁃Fe_(3)O_(4)⁃loaded CE therapeutic drugs.

关 键 词：囊型棘球蚴病 细粒棘球蚴 磁性纳米四氧化三铁 囊湿重 

分 类 号：R383.33[医药卫生—医学寄生虫学]