ATF3在PHEV感染中的表达及作用  

Expression and role of ATF3in PHEV infection

作　　者：陈雨竹 王真真 焦宇博 胡锐杰 王改丽 石俊超 贺文琦 陆慧君 高丰 宋德光 兰云刚 CHEN Yuzhu;WANG Zhenzhen;JIAO Yubo;HU Ruijie;WANG Gaili;SHI Junchao;HE Wenqi;LU huijun;GAO Feng;SONG Deguang;LAN Yungang(College of Veterinary Medicine,Jilin University,Changchun 130062,China;Jilin Province Animal Husbandry and Veterinary Academy of Sciences,Changchun 130062,China)

机构地区：[1]吉林大学动物医学学院,吉林长春130062 [2]吉林省畜牧兽医科学研究院,吉林长春130062

出　　处：《中国兽医学报》2024年第4期781-786,共6页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(32172804)。

摘　　要：为探究转录激活因子3(activating transcription factor 3,ATF3)在猪血凝性脑脊髓炎病毒(porcine hemagglutinating encephalomyelitis virus,PHEV)复制过程中的作用,本研究通过Western blot、qRT-PCR和间接免疫荧光试验检测PHEV感染后ATF3表达水平及亚细胞定位变化,并在干扰ATF3后检测PHEV含量及相关炎性细胞因子变化。结果显示,PHEV感染后,ATF3的表达水平显著上调,同时ATF3从细胞浆转入细胞核中启动下游基因表达;而干扰ATF3后,细胞内炎性因子IFN-β,IFN-γ,IL-6,IL-1β转录水平下调,PHEV含量显著增加。结果表明,PHEV感染后上调ATF3可动态调节神经元中的炎性因子并对机体产生保护作用。In order to explore the function of activating transcription factor 3(ATF3)in the porcine hemagglutinating encephalomyelitis virus(PHEV)replication process,the current investigation was carried out using Western blot,qRT-PCR,and indirect immunofluorescence assays to identify changes in ATF3expression level and subcellular localization following PHEV infection.Additionally,alterations in PHEV content and associated inflammatory cytokines were detected following ATF3interference.According to the findings,ATF3expression was dramatically increased following PHEV infection,and it translocated from the cytoplasm to the nucleus to trigger downstream gene expression.Interfering with ATF3reduced the transcription levels of inflammatory factors IFN-β,IFN-γ,IL-6,and IL-1β,while increasing the PHEV content.The results indicated that upregulation of ATF3after PHEV infection could dynamically regulate inflammatory cytokines in neurons and had a protective effect on the body.

关 键 词：猪血凝性脑脊髓炎病毒 转录激活因子3 炎性因子 病毒复制 

分 类 号：S852.3[农业科学—基础兽医学] S852.62[农业科学—兽医学]