鸭短喙矮小综合征灭活疫苗的研制及免疫效力评价  

Development and evaluation of the immune efficacy of an inactivated vaccine against duckling short beak dwarfism syndrome

作　　者：陈仕龙[1,2] 程晓霞 肖世峰[1,2] 江丹丹 林锋强 王劭 朱小丽[1,2] 俞伏松 邹敏[3] 陈少莺 CHEN Shilong;CHENG Xiaoxia;XIAO Shifeng;JIANG Dandan;LIN Fengqing;WANG Shao;ZHU Xiaoli;YU Fusong;ZOU Min;CHEN Shaoying(Institute of Animal Husbandry and Veterinary Medicine,Fujian Academy of Agricultural Sciences,Fuzhou 350000,China;Fujian Animal Diseases Control Technology Development Center,Fuzhou 350013,China;Qingdao Yebio Bioengineering Co.Ltd.,Qingdao,Shandong 266000,China)

机构地区：[1]福建省农业科学院畜牧兽医研究所,福建福州350000 [2]福建省畜禽疫病防治工程技术研究中心,福建福州350013 [3]青岛易邦生物工程有限公司,山东青岛266000

出　　处：《中国兽医学报》2024年第2期264-267,共4页Chinese Journal of Veterinary Science

基　　金：中央引导地方科技发展专项资助项目(2022L3019);福建省农业科学院“动物新发病毒病防控科技创新团队”资助项目(CXTD2021019-2);福建省农业科学院“5511”协同创新工程资助项目(XTCXGC2021012,XTCXGC2021018);福建省公益类科研院所专项资助项目(2021R10260010);福建省科技重大专题资助项目(2019NZ09008)。

摘　　要：鸭短喙矮小综合征(short beak and dwarfism syndrome,SBDS)是近年来在我国鸭群中流行的一种新传染病,为研制SBDS灭活疫苗,本研究将SBDSV M15株在番鸭胚增殖,收获病毒尿囊液经灭活后制备了3批灭活疫苗,并对疫苗的安全性、免疫原性及免疫效力进行检验。结果显示,3日龄樱桃谷鸭超剂量接种1.0 mL/羽,未见不良反应,表明疫苗的安全性好;3批灭活疫苗免疫20日龄雏半番鸭,免疫后28 d血清的胶乳凝集抑制试验(LPAI)抗体效价均值为4.3 log_(2);130日龄樱桃谷鸭种鸭免疫1次灭活疫苗后60 d血清LPAI抗体平均效价为6.9 log_(2);种鸭免疫后60 d所产种蛋卵黄抗体的LPAI效价均值为6.5 log_(2);对种鸭免疫后的3日龄子代雏鸭进行攻毒获得100%免疫保护。结果表明,本研究研制的SBDS灭活疫苗具有良好的免疫原性,种鸭1次免疫后可提供子代雏鸭保护性母源抗体,保护雏鸭度过最易感的危险窗口期,为SBDS的防控提供了有效手段。Short beak and dwarfism syndrome(SBDS)is an infectious disease prevalent in Chinese duck flocks in recent years.In order to develop a SBDS inactivated vaccine,SBDSV M15 strain was inoculated in Muscovy duck embryos,and the allantoic fluid was harvested and inactivated by formaldehyde.Three batches of oil adjuvant inactivated vaccine were prepared,and the safety,immunogenicity and immune efficacy of this vaccine was evaluated.The results showed that the vaccine has good safety to 3-day-old Cherry Valley ducklings,no any adverse reactions were observed after injection an overdose of 1 mL per duck.Twenty-day-old mule duckling were immunized with three batches of inactivated vaccine and the mean sera antibody titer detected by latex particle agglutination inhibition assay(LPAI)was 4.3 loge at 28 days post vaccination.High level of LPAI antibody was also induced in 130-day-old breed Cherry valley ducks with one dose vaccination,the average titers of LPAI antibody in sera and egg yolk were 6.9 log_(2)and 6.5 log_(2)at 60 d respectively.Complete immune protection was obtained when 3-day-old offspring ducks containing maternal antibodies were challenged with the pathogenic strain SBDSV M15.Taken together,the inactivated SBDS vaccine developed in this study has good immunogenicity,breed ducks with a single immunization could offer offspring ducklings with high level of maternal antibodies to protect the ducklings against SBDSV infection through the susceptibility period,providing an effective means for the prevention and control of SBDS.

关 键 词：鸭短喙矮小综合征 灭活疫苗 抗体效价 母源抗体 免疫效力 

分 类 号：S852.65[农业科学—基础兽医学]