人参皂苷Rg1、Re对脂多糖诱导的肠道屏障损伤的保护作用  

Protective effect of ginsenosides Rgl and Re on LPS-induced intestinal barrier damage

作　　者：张莉[1,2] 胡卫东[1] 杜林 马琪 李俊 甘玲[1,2,3] 毕师诚 ZHANG Li;HU Weidong;DU Lin;MA Qi;LI Jun;GAN Ling;BI Shicheng(College of Veterinary Medicine,Southwest University,Rongchang,Chongqing 402460,China;Immunology Research Center,Medical Research Institute,Southwest University,Rongchang,Chongqing 402460,China;National Center of Technology Innovation for Pigs,Rongchang,Chongqing 402460,China)

机构地区：[1]西南大学动物医学院,重庆荣昌402460 [2]西南大学医学研究院免疫学研究中心,重庆荣昌402460 [3]国家生猪技术创新中心,重庆荣昌402460

出　　处：《中国兽医学报》2024年第3期527-535,557,共10页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(32002325);重庆市自然科学基金资助项目(cstc2020jcyj-msxmX0418)。

摘　　要：为了探究人参皂苷Rg1、Re对脂多糖(LPS)诱导的肠道屏障损伤的保护作用,将40只4周龄雄性昆明小鼠随机分为Control、LPS、Rg1和Re组,每组10只。Control和LPS组腹腔注射无菌PBS,Rg1组腹腔注射Rg1,Re组腹腔注射Re。在试验8 d后,Control组腹腔注射无菌PBS,其余组腹腔注射LPS,6 h后处死,采样后检测。结果表明:LPS处理后DAI评分显著增加,肝脏、脾脏和肺脏指数显著升高;空肠和回肠绒毛长度显著缩短,空肠和回肠隐窝深度增加,绒毛长度与隐窝深度的比率降低;空肠和回肠紧密连接蛋白Occludin的蛋白表达显著降低;TLR4、NF-κB和Caspase-1的mRNA表达水平显著上调。而Rg1和Re预处理减轻了LPS诱导的DAI评分增加,降低了肝脏和肺脏指数;显著增加了绒毛长度,Re显著降低了空肠隐窝深度,Rg1和Re显著提高了绒毛长度与隐窝深度的比率;Rg1预处理显著增加了回肠Occludin的蛋白表达,Re预处理显著增加了空肠和回肠Occludin的蛋白表达;Rg1预处理显著下调了TLR4、NF-κB和Caspase-1的mRNA表达水平。Re预处理显著下调了TLR4和Caspase-1的mRNA表达水平。综上所述,人参皂苷Rg1、Re预处理可改善肠道形态,增加紧密连接蛋白表达,抑制炎症与凋亡相关基因的mRNA表达。To investigate the protective effects of ginsenosides Rgl and Re on LPS-induced intestinal barrier damage,404-week-old male Kunming mice were randomly divided into Control,LPS,Rgl and Re groups,with 10 mice in each group.The Control and LPS groups were injected intraperitoneally with sterile PBS,the Rg1 group was injected intraperitoneally with Rg1,and the Re group was injected intraperitoneally with Re.On the 8th day of the experiment,the Control group was injected intraperitoneally with sterile PBS,and the rest of the groups were injected intraperitoneally with LPS,mice were executed after 6 h,sampled and examined.The results showed that:after LPS treatment,DAI score increased significantly,liver,spleen and lung indices increased significantly;jejunal and ileal villus length significantly shortened,jejunal and ileal crypt depth increased,and the ratio of villus length to crypt depth decreased;the protein expression of the jejunal and ileal tight junction protein Occludin significantly decreased;the mRNAs of TLR4,NF-κB and Caspase-1 mRNA expression levels significanlly up-regulated.Whereas Rgl and Re pretreatment attenuated the LPS-induced increase in DAI scores and decreased liver and lung indices;significantly increased villus length;Re significantly decreased jejunal crypt depth,and Rg1 and Re significantly increased the ratio of villus length to crypt depth;Rg1 pretreatment significantly increased the protein expression of ileal Occludin,and Re pretreatment significantly increased jejunal and ileal Occludin;Rg1 pretreatment significantly down-regulated the mRNA expression levels of TLR4,NF-κB and Caspase-1;Re pretreatment significanlly down-regulated the mRNA expression levels of TLR4 and Caspase-1.In summary,ginsenoside Rgl and Re pretreatment improved intestinal morphology,increased tight junction protein expression,and inhibited mRNA expression of genes related to inflammation and apoptosis.

关 键 词：肠道屏障 小鼠 人参皂苷 LPS 

分 类 号：S853[农业科学—临床兽医学]