从下丘脑室旁核向背肩胛棕色组织发送投射的谷氨酸能神经元的鉴定  

Identification of glutamatergic neurons projecting from the hypothalamic paraventricular nucleus to interscapular brown adipose tissue

作　　者：曹晓娟[1] 刘昊东 李鹏辉 李嘉成 樊奇 王星 李彩琴 杨子程 郭永清[1] 陈玉洁[1] 海日汗 杜晨光[1,2,3] 张小宇 CAO Xiaojuan;LIU Haodong;LI Penghui;LI Jiacheng;FAN Qi;WANG Xing;LI Caiqin;YANG Zicheng;GUO Yongqing;CHEN Yujie;HAI Rihan;DU Chenguang;ZHANG Xiaoyu(Vocational and Technical College,Inner Mongolia Agricultural University,Baotou,Inner Mongolia 014109,China;Key Laboratory of Basic Veterinary Medicine of Inner Mongolia Autonomous Region,Hohhot O10018,China;College of Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot 010018,China)

机构地区：[1]内蒙古农业大学职业技术学院,内蒙古包头014109 [2]内蒙古自治区基础兽医学重点实验室,内蒙古呼和浩特010018 [3]内蒙古农业大学兽医学院,内蒙古呼和浩特010018

出　　处：《中国兽医学报》2024年第3期565-571,共7页Chinese Journal of Veterinary Science

基　　金：内蒙古农业大学职业技术学院草食家畜优异种质资源科技创新团队资助项目(TDY202302);国家自然科学基金资助项目(32060204);内蒙古科技攻关计划资助项目(2021GG0199,2022YFHH0015);内蒙古自治区教育厅高等学校科学研究资助项目(NJZY22535);内蒙古自治区教育厅高等学校科学研究资助项目(NJZY23120);内蒙古自治区直属高校基本科研业务费资助项目;内蒙古自治区高等学校科学研究重点资助项目(NJZZ22529)。

摘　　要：谷氨酸能神经元可抑制摄食并防止肥胖。有证据表明,在缺乏囊泡型谷氨酸转运蛋白2(VGlut2)的小鼠中,重新表达专一蛋白1(SIM1)神经元上的VGlut2可降低采食。然而,VGlut2神经元的位置和轴突投射尚不清楚。因此,本试验探寻了向背肩胛棕色脂肪组织(IBAT)发送神经元投射的上游脑区,检测了VGlut2和黑皮质素4型受体(MC4R)神经元的脑内定位,确认了胰淀素(amylin)对于VGlut2和MC4R免疫阳性神经元表达的调控作用,拓展了VGlut2神经元发送神经支配的大脑区域。结果显示,PVN^(VGlut2)神经元向IBAT发送密集的神经支配信号。在前皮质杏仁核(ACo)、纹状体(CPu)和海马齿状回(DG)发现VGlut2与MC4R共定位。amylin注射显著促进PVN^(VGlut2)::MC4R(P=0.0048)免疫阳性神经元表达,提示VGlut2与MC4R涉及amylin的调节过程。PVN^(VGlut2)神经元向下丘脑腹内侧核(VMH)、外侧下丘脑(LH)、中脑导水管周围灰质(PAG)和蓝斑(LC)核团发送神经元投射。鉴定了VGlut2神经元及下游调节能量稳态的神经环路,为探究中枢神经系统和外周组织在能量稳态调节中可能发挥的联动作用提供证据。Glutamatergic neurons inhibit food intake and prevent obesity.There is evidence that reexpression of VGlut2 on specific protein 1(SIM1)neurons can reduce food intake in mice lacking vesicular glutamate transporter 2(VGlut2).However,the location and axonal projection of VGlut2neurons are not clear.Therefore,in this study,we explored the upstream brain regions projecting to the interscapular brown adipose tissue(IBAT),detected the localization of VGlut2 and melanocortin type 4 receptor(MC4R)neurons in the brain,confirmed the regulatory effect of amylin on the expression of VGlut2 and MC4R neurons,and finally expanded the brain region where VGlut2neurons sent innervation.The results showed that PVN^(VGlut2)neurons sent dense innervation signals to IBAT.Co-localization of VGlut2 and MC4R was found in the anterior amygdaloid nucleus(ACo),Caudate putamen(CPu)and hippocampal dentate gyrus(DG).Amylin injection significantly increased the expression of PVN^(VGlut2::MC4R)neurons(P=0.0048)neurons,suggesting that VGlut2 and MC4R were involved in the regulation of amylin.PVN^(VGlut2)neurons project to the ventromedial hypothalamic nucleus(VMH),lateral hypothalamus(LH),periaqueductal gray(PAG)and locus ceruleus(LC).The identification of VGlut2 neurons and downstream neural circuits regulating energy homeostasis provides some evidence for exploring the possible linkage role of central nervous system and peripheral tissue in energy homeostasis regulation.

关 键 词：胰淀素 谷氨酸能神经元 黑皮质素4型受体 室旁核 

分 类 号：S852.1[农业科学—基础兽医学]