长吻鮠雌雄性腺转录组比较分析  

Comparative transcriptomic analysis of male and female gonads of the Chinese longsnout catfish(Leiocassis longirostris)

作　　者：樊佳慧 叶欢[2] 宋信华 岳华梅[2] 黄玲 阮瑞[2] 郜卫华[1] 李创举[2] FAN Jiahui;YE Huan;SONG Xinhua;YUE Huamei;HUANG Ling;RUAN Rui;GAO Weihua;LI Chuangju(School of Animal Science,Yangtze University,Jingzhou 434024,China;Key Laboratory of Freshwater Biodiversity Conservation,Ministry of Agriculture and Rural Affairs of China,Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Wuhan 430223,China;Hubei Product Farm of Leiocassis longirostris,Shishou 434400,China)

机构地区：[1]长江大学动物科学学院,湖北荆州434024 [2]中国水产科学研究院长江水产研究所,农业农村部淡水生物多样性保护重点实验室,湖北武汉430223 [3]湖北省长吻鮠良种场,湖北石首434400

出　　处：《中国水产科学》2024年第2期129-143,共15页Journal of Fishery Sciences of China

基　　金：国家重点研发计划子课题(2022YFD400101);中国水产科学研究院中央级公益性科研院所基本科研业务费专项资金资助(2022XT03和2023TD03);中央级公益性科研院所基本科研业务费专项资金资助(YFI202206);贵州省科技计划项目([2020]4Y027)。

摘　　要：长吻鮠(Leiocassislongirostris)是淡水特色经济鱼类,其雄性生长速度快于雌性,培育全雄苗种可显著提高养殖效益。为加快长吻鮠全雄苗种培育进程,需深入了解长吻鮠性别分化及性腺发育的调控机制。本研究通过Illumina高通量测序平台对长吻鮠卵巢和精巢进行转录组测序分析。结果显示,共有10872个雌雄差异表达基因,其中上调基因9375个,下调基因1497个。通过功能注释,筛选出71个与性别相关的重要候选基因,包括50个精巢高表达基因(dmrt1、cyp17a1、samd7、wnt6、wt1等)和21个卵巢高表达基因(foxl2、gdf9、zp3、zp1、figla、bmp15等)。KEGG通路富集分析发现,差异表达基因显著富集到16条与性别决定与分化及性腺发育相关的信号通路,包括Wnt信号通路、TGF-β信号通路、MAPK信号通路、卵巢类固醇通路、GnRH信号通路等。本研究筛选出与长吻鮠性别决定和分化相关差异表达基因,揭示参与其雌雄个体性腺发育的信号通路,为今后长吻鮠性别决定和分化机制的研究积累重要数据,从而为其全雄苗种的培育提供理论支撑。The Chinese longsnout catfish(Leiocassis longirostris)is an economically important freshwater fish in China;males grow faster than the females,suggesting that all-male production is economically desirable in aquaculture.However,their sex determination,sex differentiation genes,and the related regulatory mechanisms remain unknown.In this study,transcriptome sequencing of the ovaries and testes of Chinese longsnout catfish was performed using an Illumina high-throughput sequencing platform.We obtained 358048456 raw reads and 356168472 clean reads from the transcriptome.In total,24661 genes were annotated,of which 23708 genes were aligned with the reference genome.Gene expression patterns in the ovaries and testes were compared.The results showed that there were 10872 differentially expressed genes(DEGs)between the ovary and testis,including 9375 up-regulated genes and 1497 down-regulated genes.21 functional DEGs were selected for RT-qPCR analysis and the results confirmed the reliability of the transcriptome analysis.The DEGs were annotated using the Nr,KEGG,and GO databases,where they were enriched in 58 GO functional annotation components and 338 KEGG secondary branching metabolic systems.Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis revealed that the differentially expressed genes were significantly enriched in 16 pathways related to sex determination and differentiation and gonadal development,including the Wnt signaling,TGF-βsignaling,MAPK signaling,ovarian steroid signaling,and GnRH signaling pathways.Through functional annotation,71 important sex-related candidate genes were screened,including 50 highly expressed genes(such as dmrt1,cyp17a1,samd7,wnt6,and wt1)and 21 highly expressed ovarian genes(such as foxl2,gdf9,zp3,zp1,figla,and bmp15).Protein–protein interaction(PPI)network analysis revealed that the testis specificity gene dmrt1 interacts with the testis-biased DEGs(sox9,wt1,cyp17a1,rspo1,gata4,and hsd3b)and the ovary-biased DEGs(foxl2,figla,and bmp1),suggesting that dmrt1

关 键 词：长吻鮠 转录组 性腺发育 差异表达基因 

分 类 号：S917[农业科学—水产科学]