Classical taxonomy,molecular phylogeny and genetic analysis of the genus Exitianus Ball,1929(Hemiptera:Cicadellidae:Deltocephalinae)from Egypt  

作　　者：Rabab M.El-Hady Ahmed F.El-Sayed 

机构地区：[1]Survey&Taxonomy Department,Plant Protection Research Institute,Agriculture Research Centre,Giza,Egypt [2]Microbial Genetics Department,Biotechnology Research Institute,National Research Centre,Giza,Egypt [3]Egypt Center for Research and Regenerative Medicine(ECRRM),Cairo,Egypt

出　　处：《Zoological Systematics》2024年第2期143-157,共15页动物分类学报（英文版）

摘　　要：Egyptian species of the leafhopper genus Exitianus Ball,1929,E.capicola(St?l,1855),E.nanus(Distant,1908),and E.pondus Ross,1968 are reviewed.Illustrations,morphological descriptions,and a key for their identification are provided.In this study,we used molecular techniques to confirm morphological identification and detect phylogeny among the three Exitianus species.The partial nucleotide sequences of the amplified products obtained were determined by Macrogen Korea.The nucleotide sequences of 28S rDNA and COX genes of the EGY-ARC-9,EGY-ARC-4,and EGY-ARC-5 were determined by Macrogen Korea,blasted into BLAST at the National Center for Biotechnology Information website(NCBI)and compared with those deposited in the GenBank DNA database.The results represented the homology percentage between the partial sequences of the 28S rRNA and COX genes from each species and related species obtained from GenBank DNA database.The morphological identifications of the three Exitianus species were confirmed by molecular characterization and sequencing of 28S rDNA and COX genes and identified as E.capicola,E.nanus,and E.pondus.Also,their sequences of 28srDNA and COX genes were deposited in GenBank with an accession number(LC670610,LC670607,and OQ196105)for 28srDNA gene and(LC775357,LC775358,and LC775359)for the COX gene.

关 键 词：AUCHENORRHYNCHA Chaismini TAXONOMIC 28S rDNA phylogenetic 

分 类 号：Q969[生物学—昆虫学]