一测多评法同时测定连翘和连翘叶中多种成分  被引量:2

Simultaneous quantitative determination of 7 components in Forsythiae Fructus and Forsythia suspense leaves by QAMS method

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作  者:田淑芳 韩旭 谭奥飞 王安 周园园 王梦夕 孟晨晨 程铁峰[1] TIAN Shufang;HAN Xu;TAN Aofei;WANG An;ZHOU Yuanyuan;WANG Mengxi;MENG Chenchen;CHENG Tiefeng(School of Pharmacy,Henan University,Kaifeng 475004,CHN)

机构地区:[1]河南大学药学院,河南开封475004

出  处:《河南大学学报(医学版)》2024年第2期96-102,共7页Journal of Henan University:Medical Science

基  金:国家中医药管理局中药标准化(ZYBZH-Y-HEN-19)。

摘  要:目的:建立同时测定连翘和连翘叶中绿原酸、芦丁、连翘酯苷B、连翘酯苷A、松脂醇-β-D-葡萄糖苷、连翘苷和连翘酯素7种成分的一测多评(QAMS)方法。方法:采用HPLC法,以连翘苷为内参物,分别建立连翘苷与其余6种成分的相对校正因子(RCF),并计算含量,实现一测多评;同时采用外标法测定该7种成分的含量,比较两种方法的差异,验证QAMS的准确性和可行性。结果:在不同实验条件下,RCF重现性良好,13批样品中该7种成分的一测多评值和外标法测定值无显著差异。结论:QAMS法可用于连翘和连翘叶的定量分析。Objective:To establish the quantitative analysis of multir components with a single marker(QAMS)for simultaneously determination of seven components(Chlorogenic acid,rutin,forsythoside B,forsythoside A.pinoresinol-B-D-glucoside,phillyrin,phillygenin)in Forsythiae Fructus and Forsythia suspense leaves.Methods:The relative correction factors(RCF)of phillyrin and the other six components were established by HPLC with pillyrin as the internal reference,and the content was calculated to realize QAMS;At the same time,the contents of the seven components were determined by external standard method,and the differences between the two methods were compared.to verify the accuracy and feasibility of QAMS.Results:Under different experimental conditions,the reproducibility of the relative correction factor is well,there was no significant difference between the measured values of QAMS method and ESM for the contents of the seven components in 13 batches of samples.Conclusion:QAMS is accurate and feasible to evaluate the quality of Forsythia suspensa and Forsythia suspensa leaves.

关 键 词:一测多评法 连翘 连翘叶 相对校正因子 

分 类 号:R927.2[医药卫生—药学]

 

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