慢性丙型肝炎患者CD14^(+)单核细胞和CD163^(+)巨噬细胞的表达及其与肝纤维化严重程度的关系  

Expression of CD14^(+)monocytes and CD163^(+)macrophages in patients with chronic hepatitis C and their relationship with the severity of liver fibrosis

作　　者：贺宇[1] 张志红[2] 欧阳慧 鲁石[1] HE Yu;ZHANG Zhi-hong;OUYANG Hui;LU Shi(epartment of Laboratory,Gong'an County People's Hospital Hubei 434300,China;Department of Oncology,Gong'an County People's Hospital Hubei 434300,China;Department of Blood Transfusion,Gong'an County People's Hospital Hubei 434300,China)

机构地区：[1]公安县人民医院检验科,湖北434300 [2]公安县人民医院肿瘤科,湖北434300 [3]公安县人民医院输血科,湖北434300

出　　处：《肝脏》2024年第4期432-435,共4页Chinese Hepatology

基　　金：湖北省卫生健康委员会科研项目(WJ2023M177)。

摘　　要：目的探讨慢性丙型肝炎患者CD14^(+)单核细胞和CD163^(+)巨噬细胞的表达及其与肝纤维化严重程度的关系。方法选取2018年4月—2022年11月期间公安县人民医院诊治的慢性丙型肝炎患者83例,同期健康人64例,比较两组临床特征、肝组织中CD163表达、血清CD163浓度、CD14^(+)细胞比例以及表达不同种类细胞因子的CD14^(+)细胞比例。结果慢性丙型肝炎组(n=82)ALT水平和AST水平分别为(84.6±9.1)U/L和(69.3±7.2)U/L,显著高于健康对照组(n=64)的(28.4±1.7)U/L和(22.5±6.9)U/L(P<0.05);慢性丙型肝炎患者中F<2者CD163^(+)细胞数量、血清CD163浓度和CD14^(+)细胞比例分别为38.2±4.7、(73.6±2.1)μg/L和(73.7±3.2)%,显著高于健康对照组的12.5±1.3、(50.1±2.6)μg/L和(61.0±8.4)%,F≥2患者CD163^(+)细胞数量、血清CD163浓度和CD14^(+)细胞比例分别为74.9±8.0、(95.4±9.8)μg/L和(79.2±5.5)%,显著高于健康对照组和F<2患者。F<2慢性丙型肝炎患者表达IL-2、IL-4、IFN-γ、IL-6、IL-8和TNF-α的CD14^(+)细胞比例分别为(4.2±1.2)%、(3.8±1.1)%、(3.8±1.0)%、(3.7±1.3)%、(4.0±1.4)%和(6.7±3.6)%,显著高于健康对照组的(0.9±0.1)%、(1.1±0.2)%、(1.8±0.4)%、(1.6±0.6)%、(1.7±0.7)%和(4.6±2.1)%,F≥2患者表达IL-2、IFN-γ、IL-8和TNF-α的CD14^(+)细胞比例分别为(2.2±0.8)%、(2.1±0.5)%、(5.6±2.0)%和(6.9±3.2)%,显著高于健康对照组,表达IL-8的CD14^(+)细胞比例为(5.6±2.0)%,显著高于F<2患者。结论CD14^(+)单核细胞和CD163^(+)巨噬细胞参与了慢性丙型肝炎患者肝纤维化的进程。Objective To investigate the expression of CD14^(+)monocytes and CD163^(+)macrophages in patients with chronic hepatitis C and their relationship with the severity of liver fibrosis.Methods A total of 83 patients with chronic hepatitis C and 64 healthy subjects were selected between April 2018 and November 2022.The clinical features,the expression of CD163 in liver tissue,the concentration of serum CD163,the proportion of CD14^(+)cells and the proportion of CD14^(+)cells expressing different kinds of cytokines were compared.Results The level of ALT and AST of chronic hepatitis C group were(84.6±9.1)U/L and(69.3±7.2)U/L,which was significantly higher than that in healthy control group[(28.4±1.7)U/L and(22.5±6.9)U/L](P<0.05).The concentration of serum CD163 and the proportion of CD14^(+)cells,the number of CD163^(+)cells,concentration of serum CD163^(+)and CD14^(+)cell proportion of F<2 patients were 38.2±4.7,(73.6±2.1)μg/L and(73.7±3.2)%,which was significantly higher than that in healthy control group[12.5±1.3,(50.1±2.6)?g/L and(61.0±8.4)%](P<0.05).The number of CD163^(+)cells,concentration of serum CD163^(+)and CD14^(+)cell proportion of F≥2 patients were 74.9±8.0,(95.4±9.8)μg/L and(79.2±5.5)%,which was significantly higher than that in healthy control group and F<2 patients.CD14^(+)cells expressing IL-2,IL-4,IFN-γ,IL-6,IL-8 and TNF-αof F<2 patients were(4.2±1.2)%,(3.8±1.1)%,(3.8±1.0)%,(3.7±1.3)%,(4.0±1.4)%and(6.7±3.6)%,which was significantly higher than that in healthy control group[(0.9±0.1)%,(1.1±0.2)%,(1.8±0.4)%,(1.6±0.6)%,(1.7±0.7)%and(4.6±2.1)%]P<0.05).CD14^(+)cells expressing IL-2,IFN-γ,IL-8 and TNF-αof F≥2 patients were(2.2±0.8)%,(2.1±0.5)%,(5.6±2.0)%and(6.9±3.2)%,which was significantly higher than that in healthy control group.CD14^(+)cells expressing IL-8 was(5.6±2.0)%,which was significantly higher than that in F≥2 patients.Conclusion CD14^(+)monocytes and CD163^(+)macrophages are involved in the process of liver fibrosis in patients with chronic hepatitis C

关 键 词：慢性丙型肝炎 单核细胞 巨噬细胞 

分 类 号：R512.63[医药卫生—内科学] R575.2[医药卫生—临床医学]