鲤在阿维菌素胁迫下肝胰腺组织的转录组分析  

Transcriptome Analysis of Hepatopancreas Tissue in Common Carp Exposed to Abamectin Stress

作　　者：李兴洋 乔璐 杨臻 徐锦华 宋金龙[2] 孙慧武[2] 穆迎春[2] LI Xingyang;QIAO Lu;YANG Zhen;XU Jinhua;SONG Jinlong;SUN Huiwu;MU Yingchun(College of Food Science and Engineering,Bohai University,Jinzhou 121000,China;Key Laboratory of Control of Quality and Safety for Aquatic Products,Ministry of Agriculture and Rural Affairs,Chinese Academy of Fishery Sciences,Beijing 100141,China)

机构地区：[1]渤海大学食品科学与工程学院,辽宁锦州121000 [2]中国水产科学研究院农业农村部水产品质量安全控制重点实验室,北京100141

出　　处：《水产学杂志》2024年第2期26-32,45,共8页Chinese Journal of Fisheries

基　　金：国家农产品质量安全风险评估专项项目(GJFP20210401);虾蟹及淡水鱼质量安全风险评估与防控创新团队项目(2020TD75)。

摘　　要：为了探究阿维菌素胁迫对鲤机体的响应机制,在水温(22±2.0)℃,将体质量(150±30)g的鲤(Cyprinus carpio)分别暴露在阿维菌素浓度0μg·L^(-1)(对照组)、1.5μg·L^(-1)和3.0μg·L^(-1)下5 d,采用转录组学测序分析方法,探究阿维菌素胁迫对鲤肝胰腺转录组学的影响,解析其对鲤的分子毒理机制。通过对所得基因的功能注释发现,被注释的差异基因主要与结合、催化和代谢等功能有关。KEGG通路富集分析结果显示,差异表达基因在药物代谢-细胞色素P450、药物代谢-其他酶、淀粉和蔗糖代谢等通路中显著富集,涉及药物代谢、氨基酸代谢、碳水化合物代谢、脂质代谢以及辅助因子和维生素的代谢等多个代谢过程。这些功能基因和预测通路为理解阿维菌素胁迫鲤体内解毒和免疫系统奠定了基础。本研究获得的转录组数据可为深入研究鱼类应对杀虫剂污染物的分子机制提供丰富的基因资源。In order to explore response mechanism of common carp(Cyprinus carpio)to abamectin stress,the common carps with body weight of(150±30)g were exposed to abamectin under concentrations of 0μg·L^(-1)(control group),1.5μg·L^(-1) and 3.0μg·L^(-1) for 5 days at a water temperature of(22±2.0)℃to investigate the effect of abamectin stress on the transcriptomics of hepatopancreas tissue in common carp by transcriptome sequencing analysis method,and analyze its molecular toxicological mechanism to carp.Functional annotation of obtained genes showed that the annotated differential genes were mainly related to the functions of binding,catalysis and metabolism.The enrichment analysis results of KEGG pathway indicated that the differentially expressed genes(DEGs)were significantly enriched in drug metabolism-cytochrome P450,drug metabolism-other enzymes,starch and sucrose metabolism and other pathways,involved in drug metabolism,amino acid metabolism,carbohydrate metabolism,lipid metabolism,cofactor and vitamin metabolism and other metabolic processes.These functional genes and predictive pathways laid the foundation for understanding the detoxification and immune system of common carp exposed to abamectin stress.The obtained transcriptome data provided rich gene resources for further research on the molecular mechanism of fish response to insecticide pollutants.

关 键 词：鲤 阿维菌素 转录组学 肝胰腺 

分 类 号：S859.84[农业科学—临床兽医学] S965.116[农业科学—兽医学]