石榴皮提取物对副溶血弧菌的抑制效应与机制研究  

Inhibitory Effect and Mechanism of Pomegranate Peel Aqueous Extract on Vibrio parahaemolyticus

作　　者：司梦雅 赵宏晶 郝俊 袁春营 崔青曼 SI Mengya;ZHAO Hongjing;HAO Jun;YUAN Chunying;CUI Qingman(College of Ocean and Environment,Tianjin University of Science and Technology,Tianjin 300457,china;Tianjin Fisheries Research Institute,Tianjin 300210,China)

机构地区：[1]天津科技大学海洋与环境学院,天津300457 [2]天津市水产研究所,天津300210

出　　处：《水产学杂志》2024年第2期70-76,共7页Chinese Journal of Fisheries

基　　金：天津市科技计划项目(20YFZCSN00140);天津市现代农业产业技术体系创新团队(ITTMRS2021009)。

摘　　要：研究了二次煎煮的石榴皮提取物(Pomegranate peel aqueous extract,PPAE)对副溶血弧菌(Vibrio parahaemolyticus)的抑制活性作用及其机理,为防治水产养殖弧菌病提供理论依据。采用二倍稀释法测定石榴皮提取物对副溶血弧菌的最小抑菌浓度(MIC)和最小杀菌浓度(MBC)、电导率仪检测弧菌的相对电导率、分光光度法测定弧菌的核酸泄露及生物膜抑制率、蛋白质电泳检测弧菌的蛋白质合成、扫描电子显微镜观察弧菌的亚显微结构及碘化丙啶(PI)染色分析弧菌细胞膜完整性。结果表明:PPAE对副溶血弧菌具有明显的抑制作用,MIC和MBC分别为1.563 mg·m L^(-1)和3.125 mg·m L^(-1);PPAE能够显著抑制副溶血弧菌生物膜形成(P<0.05);显著增加副溶血弧菌的核酸泄露和电导率(P<0.01),明显抑制副溶血弧菌蛋白合成;明显改变副溶血弧菌亚显微形态,破坏副溶血弧菌细胞膜的完整性。The inhibitory activity and mechanism of pomegranate peel aqueous extract(PPAE)against Vibrio parahaemolyticus were investigated in order to provide theoretical basis for the prevention and treatment of vibriosis in aquaculture.The minimum inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)of PPAE against V.parahaemolyticus were measured by double dilution method,and the relative conductivity was detected by conductivity meter.The leakage of nucleic acid and the biofilm inhibition rate of V.parahaemolyticus were determined by spectrophotometry,the protein synthesis of V.parahaemolyticus was detected by protein electrophoresis,the submicrostructure of V.parahaemolyticus was observed by scanning electron microscope,and the integrity of cell membrane in V.parahaemolyticus was analyzed by propidium iodide(PI)staining.The results showed that PPAE had obvious inhibitory effect on V.parahaemolyticus,with the MIC of 1.563 mg·mL^(-1) and MBC of 3.125 mg·mL^(-1),indicating that PPAE significantly inhibits the biofilm formation of V.parahaemolyticus(P<0.05).After PPAE treatment,nucleic acid leakage and relative conductivity of V.parahaemolyticus wereshown to be increased significantly(P<0.01),and the protein synthesis of Vibrio parahaemolyticus was found to be significantly inhibited.PPAE obviously changed the submicroscopic morphology and destroyed the cell membrane integrity of V.parahaemolyticus.

关 键 词：石榴皮提取物 副溶血弧菌 抑菌机制 

分 类 号：Q939.1[生物学—微生物学]