流式细胞术检测药用植物基因组大小的研究策略  

Research Strategy of Flow Cytometry Detection of Genome Size of Medicinal Plants

作　　者：刘佩 杜芬 邓静怡 曾蒋玲 卢小舒 蓝培基 LIU Pei;DU Fen;DENG Jingyi;ZENG Jiangling;LU Xiaoshu;LAN Peiji(Shaoguan University,Shaoguan 512026,China)

机构地区：[1]韶关学院,广东韶关512026

出　　处：《医学综述》2024年第11期1292-1295,共4页Medical Recapitulate

基　　金：广东省省级大学生创新创业训练计划项目(S202110576065)。

摘　　要：流式细胞术是一种可以对悬浮于液体中微小颗粒进行分选和计数的方法。该方法的步骤包括制备完整的细胞核悬浮液、对核酸荧光染色、根据相关荧光含量分类。分析荧光标记悬液中的单细胞或其他微小颗粒信号,可以实现蛋白质含量、细胞DNA含量、酶活性、细胞膜受体和表面抗原、细胞体积等参数的快速测定。该技术可以用于植物倍性分析和基因组大小估测等研究。目前利用流式细胞术对药用植物进行基因组大小评估的研究较少,未来有待深入研究,以期为利用流式细胞术检测基因组大小提供相关资料。Flow cytometry is a method for sorting and counting tiny particles suspended in liquid.The steps of this method include the preparation of complete nuclear suspension,fluorescence staining of nucleic acids,and classification according to the relevant fluorescence content.By analyzing the single cell or other microparticle signals in the fluorescent labeling suspension,parameters such as protein content,cell DNA content,enzyme activity,cell membrane receptors and surface antigens,and cell volume can be quickly determined.This technology can be applied to plant ploidy analysis and genome size estimation.At present,there are few studies on flow cytometry to evaluate the genome size of medicinal plants,and further research is needed in the future to provide relevant data on genome size detection by flow cytometry.

关 键 词：流式细胞术 药用植物 C值 DNA含量 

分 类 号：Q943.2[生物学—植物学]