白术多糖提取分离工艺研究  

Study on Extraction and Separation Process of Polysaccharide from Baizhu(Atractylodes macrocephala)

作　　者：彭静 潘东梅 潘东莲 史哲铭 袁婧玮 曾雄龙 黄裕成 易延逵[2] PENG Jing;PAN Dongmei;PAN Donglian;SHI Zheming;YUAN Jingwei;ZENG Xionglong;HUANG Yucheng;YI Yankui(Xiangxi Autonomous Prefecture People's Hospital,The First Affiliated Hospital of Jishou University,Jishou 416000,Hunan,China;School of Traditional Chinese Medicine of Southern Medical University,Guangzhou 510515,Guangdong,China)

机构地区：[1]湘西自治州人民医院,吉首大学第一附属医院,湖南吉首416000 [2]南方医科大学中医药学院,广东广州510515

出　　处：《中华中医药学刊》2024年第5期41-45,I0016,共6页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(82004101);广东省创新训练项目(X202012121374)。

摘　　要：目的优选白术多糖的提取分离工艺。方法比较氯仿-正丁醇Sevage法去蛋白与不去蛋白白术多糖含量。以吸附率和解吸率为考察指标,优选AB-8型、D101型、LX-38型3种大孔吸附树脂。通过吸附时间、解吸时间、上样流速、上样浓度、洗脱剂用量、洗脱剂浓度和洗脱流速研究AB-8型大孔树脂纯化白术多糖工艺。结果未去蛋白的多糖含量为(5.582±1.4480)%、(5.407±0.7668)%、(5.155±0.7214)%,Sevage法去蛋白后的多糖含量为(4.658±0.1558)%、(4.247±0.0742)%、(4.610±0.1734)%。AB-8型大孔树脂较其他两种树脂,其吸附率及解吸率可达(18.40±0.0300)%,(91.73±0.0781)%,纯化工艺为吸附时间3 h、解吸时间3 h、上样流速1 BV/h、上样浓度0.75 mg/mL、洗脱剂用量4 BV、洗脱剂浓度15%乙醇、洗脱流速3 BV/h。结论本试验为白术多糖的提取分离提供了试验依据,为白术多糖的工业化生产提供了参考。Objective To optimize the extraction and separation process of polysaccharides from Baizhu(Atractylodes macro⁃cephala).Method The contents of polysaccharides from Baizhu(Atractylodes macrocephala)without protein removal and protein removal by Sevage method were compared.Three macroporous adsorption resins of AB-8,D101 and LX-38 were investigated based on adsorption rate and desorption rate.The purification process of AB-8 macroporous adsorption resin was studied by ad⁃sorption time,desorption time,sample flow rate,sample concentration,eluent dosage,eluent concentration and elution flow rate.Result The contents of polysaccharides without removing protein were(5.582±1.4480)%,(5.407±0.7668)%and(5.155±0.7214)%.The contents of polysaccharides after removing protein by Sevage method were(4.658±0.1558)%,(4.247±0.0742)%and(4.610±0.1734)%.The adsorption rate and desorption rate of AB-8 macroporous resin were(18.40±0.0300)%and(91.73±0.0781)%respectively.The purification process was as follows:adsorption time 3 h,de⁃sorption time 3 h,sample flow rate 1 BV/h,sample concentration 0.75 mg/mL,eluent dosage 4 BV,eluent concentration 15%ethanol,elution flow rate 3 BV/h.Conclusion The experiment provided the experimental basis for the extraction and separation of and a reference for the industrial production of polysaccharides from Baizhu(Atractylodes macrocephala).

关 键 词：白术 多糖 提取 分离 大孔吸附树脂 

分 类 号：R284.2[医药卫生—中药学]