青天葵总黄酮对人鼻咽癌CNE-2荷瘤裸鼠皮下肿瘤的抑制作用  

The tnhibitory effect of Total Flavonoids from Nervilia Fordii on subcutaneous tumors of human nasopharyngeal carcinoma CNE-2 in tumor-bearing nude mice

作　　者：秦晓辉 刘智强 刘俊阳 黄春想 王可盈 宋家乐 周燕园[1,5] QIN Xiaohui;LIU Zhiqiang;LIU Junyang;HUANG Chunxiang;WANG Keying;SONG Jiale;ZHOU Yanyuan(School of Pharmacy,Guilin Medical University,Guilin 541199,China)

机构地区：[1]桂林医学院药学院,广西桂林541199 [2]桂林医学院公共卫生学院,广西桂林541199 [3]广西环境暴露组学与全生命周期健康重点实验室,广西桂林541101 [4]桂林理工大学国家母婴乳品健康工程技术研究中心南亚分中心,广西桂林541004 [5]广西药物分子发现与成药性优化重点实验室广西药物分子筛选与成药性评价工程研究中心,广西桂林541199

出　　处：《陕西中医》2024年第5期593-599,共7页Shaanxi Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(82360854);广西壮族自治区自然科学基金资助项目(2022GXNSFAA035603);广西药物分子发现与成药性优化重点实验室开放课题资助项目(GKLPMDDO-2022-C01)。

摘　　要：目的:研究青天葵总黄酮(TFNF)对BALB/c裸鼠人鼻咽癌细胞皮下种植模型中TGF-β1/Erk/MAPK通路的调控作用。方法:BALB/c裸鼠皮下接种CNE-2细胞(1.0×107细胞/ml)建立鼻咽癌荷瘤模型,苏木精-伊红染色(HE)行病理判断,免疫组化法(IHC)观察细胞周期调节蛋白-67(Ki67)、增殖细胞核抗原(PCNA)的表达水平。免疫印迹法检测转化生长因子-β1(TGF-β1)、细胞外调节蛋白激酶(Erk)、p-Erk、丝裂原活化蛋白激酶(MAPK)、p-MAPK的蛋白表达水平。试剂盒法评估肾脏、肝脏、脾脏中超氧化物歧化酶(SOD)及丙二醛(MDA)水平。结果:与模型组比较,高剂量TFNF显著抑制荷瘤小鼠肿瘤体积与质量,显著降低Ki67(55.19%)、PCNA(46.65%)表达水平,提高肾脏、肝脏、脾脏中SOD水平至3.88、2.98、3.71U/mgprot,降低肾脏、肝脏、脾脏的MDA水平至2.19、1.05、2.85nmol/mgprot。TFNF可降低肿瘤组织中TGF-β1(47.36%)、p-Erk/Erk(81.13%)、p-MAPK/MAPK(19.80%)的蛋白表达水平。结论:TFNF可调控TGF-β1/Erk/MAPK通路抑制肿瘤生长,并减轻荷瘤裸鼠主要脏器的氧化损伤发生。Objective:The regulatory effect of Total Flavonoids from Nervilia fordii(TFNF)on the TGF-β1/Erk/MAPK pathway in the subcutaneous transplantation model of human nasopharyngeal cancer cells in BALB/c nude mice was investigated.Methods:BALB/c nude mice were inoculated with CNE-2 cells(1.0×107cells/ml)to establish a tumor-bearing model of nasopharyngeal carcinoma,the pathological determination was performed by hematoxylin-eosin(HE),and the expression of cell cycle regulatory protein-67(Ki67)and proliferation nuclear antigen(PCNA)were observed by immunohistochemistry(IHC).Western blotting detected the protein expression of transforming growth factor-β1(TGF-β1),extracellular regulated protein kinase(Erk),p-Erk,mitogen-activated protein kinase(MAPK),and p-MAPK.Superoxide dismutase(SOD)and malondialdehyde(MDA)levels in the kidneys,liver,and spleen were assessed by the kit.Results:Compared with the model group,high-dose TFNF significantly inhibited tumor volume and weight,significantly reduced the expression levels of Ki67(55.19%)and PCNA(46.65%),increased SOD levels in kidneys,liver,and spleen(to 3.88,2.98,3.71 U/mg prot),and decreased MDA levels in kidneys,liver,and spleen(to 2.19,1.05,2.85 nmol/mg prot).In addition,TFNF can also reduce the protein expression levels of TGF-β1(47.36%),p-Erk/Erk(81.13%),and p-MAPK/MAPK(19.80%)in tumor tissues.Conclusion:The results showed TFNF could regulate the TGF-β1/Erk/MAPK pathway,inhibit tumor growth,and reduce the occurrence of oxidative damage to the main organs of tumor-bearing nude mice.

关 键 词：鼻咽癌 青天葵总黄酮 TGF-β1/Erk/MAPK信号通路 CNE-2细胞 BALB/C裸鼠 氧化损伤 

分 类 号：R766.3[医药卫生—耳鼻咽喉科]