高粱CIPK家族基因的全基因组鉴定及非生物胁迫下的表达特征  

Whole genome-wide identification of CIPK family and their expression characteristics under abiotic stress in Sorghum bicolor

作　　者：徐鹏[1] 李春宏[1] 范昕琦 梁笃[2,3] 沈新莲 XU Peng;LI Chun-hong;FAN Xin-qi;LIANG Du;SHEN Xin-lian(Institute of Industrial Crops,Jiangsu Academy of Agricultural Sciences/Key Laboratory of Cotton and Rapeseed,Ministry of Agriculture and Rural Affairs,Nanjing 210014,China;College of Agriculture,Shanxi Agricultural University,Jinzhong 030801;Shanxi Key Laboratory of Sorghum Genetic and Germplasm Innovation,Sorghum Research Institute of Shanxi Academy of Agricultural Sciences,Jinzhong 030600)

机构地区：[1]江苏省农业科学院经济作物研究所/农业农村部长江下游棉花与油菜重点实验室,江苏南京210014 [2]山西农业大学农学院,山西晋中030801 [3]山西省农业科学院高粱研究所/高粱遗传与种质创新山西省重点实验室,山西晋中030600

出　　处：《江苏农业学报》2024年第4期591-598,共8页Jiangsu Journal of Agricultural Sciences

基　　金：亚夫科技服务项目[KF(21)3001];山西省基础研究计划项目(202103021223130);山西种业创新良种联合攻关项目(2022N2GL-06);山西省农业科学院杂粮分子育种平台专项(YGC2019FZ5);山西省科技合作交流专项(202204041101032)。

摘　　要：钙调磷酸酶B样蛋白互作蛋白激酶(CIPK)是一种重要的Ca^(2+)信号传感器,在植物应答逆境非生物胁迫过程中发挥着重要作用。为了探究高粱中CIPK家族基因的功能,本研究从高粱基因组中鉴定了31个SbCIPK基因,这些基因不均匀地分布在高粱的9条染色体上,编码蛋白质的氨基酸数量为403~519个,等电点为6.07~9.38,相对分子质量为46 357.31~58 316.97。基因结构分析结果表明,SbCIPK家族基因分为内含子缺失型和内含子富集型2类。进化树分析结果表明,SbCIPK家族蛋白质成员分为8个亚族。基于转录组数据的表达模式分析结果表明,SbCIPK基因广泛参与对盐胁迫、干旱胁迫等非生物胁迫的响应。本研究结果可以为高粱CIPK家族基因的功能研究奠定基础。Calcineurin B-like proteins interacting protein kinase(CIPK)is an important Ca^(2+)signal sensor,which plays an important role in plants’response to abiotic stress.In order to explore the function of CIPK family gene members in Sorghum bicolor’a total of 31 SbCIPK genes were identified,which were unevenly distributed on nine chromosomes from Sorghum bicolor in this study.The length of the SbCIPKs encoded proteins ranged from 403 to 519 aa,the isoelectric points ranged from 6.07 to 9.38,and the relative molecular weights ranged from 46357.31 to 58316.97.The SbCIPK gene family members were divided into intron deletion type and intron enrichment type by gene structure analysis.According to the results of phylogenetic tree analysis,the S.bicolor SbCIPK family protein members were divided into eight subgroups.SbCIPK genes were widely involved in abiotic stress responses to salt and drought and so on based on the published transcriptome data.These results can lay the foundation for the functional study of SbCIPK family members in S.bicolor.

关 键 词：高粱 CIPK基因 全基因组鉴定 非生物胁迫 

分 类 号：S514[农业科学—作物学]