噬菌体裂解酶基因Lys BT1在普通小球藻中的表达  

Expression of bacteriophage lyase gene Lys BT1 in Chlorella vulgaris

作　　者：束忠涛 王冉[2] 沈元朝 张汉泽 朱树娇 王姝璇 孙利厂[2] SHU Zhong-tao;WANG Ran;SHEN Yuan-chao;ZHANG Han-ze;ZHU Shu-jiao;WANG Shu-xuan;SUN Li-chang(School of Food and Biological Engineering,Jiangsu University,Zhenjiang 212013,China;Institute of Food Safety and Nutrition,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China)

机构地区：[1]江苏大学食品与生物工程学院,江苏镇江212013 [2]江苏省农业科学院农产品质量安全与营养研究所,江苏南京210014

出　　处：《江苏农业学报》2024年第4期734-739,共6页Jiangsu Journal of Agricultural Sciences

基　　金：江苏省农业科技自主创新基金项目[CX(22)2017];江苏省政策引导类计划一带一路创新合作项目(BZ2021009);国家重点研发计划项目(2021YFE0101800)。

摘　　要：噬菌体裂解酶因高效、安全的杀菌特性成为潜在的抗菌药物,并受到广泛关注。本试验在前期通过基因表达制备高效噬菌体裂解酶Lys BT1的基础上,深度解析Lys BT1与细菌上裂解酶受体相互作用的结构特点,成功构建出质粒pCAMBIA 1301-BT1。将该质粒通过电转化的方式导入普通小球藻中,电转条件为:电场强度1.5 kV、脉冲距离2 mm、脉冲时间0.2 ms,瞬时表达后成功进行了活性测定,从而验证了普通小球藻作为裂解酶表达载体的可行性,为噬菌体裂解酶的表达系统研发提供了一条可行路径。Bacteriophage lyase has become a potential antibacterial drug due to its high efficiency and safe bactericidal properties,and has been widely concerned.In this study,based on the preparation of high-efficiency bacteriophage lyase Lys BT1 by gene expression in the early stage,we deeply analyzed the structural characteristics of Lys BT1 interacting with the lyase receptor on bacteria,and successfully constructed the plasmid pCAMBIA 1301-BT1.The plasmid was introduced into Chlorella vulgaris by electrotransformation.The electrotransformation conditions were as follows:electric field intensity of 1.5 kV,pulse distance of 2 mm,and pulse time of 0.2 ms.After transient expression,the activity determination was successfully carried out.Therefore,the feasibility of Chlorella vulgaris as a lyase expression vector was verified,which provided a feasible path for the development of bacteriophage lyase expression system.

关 键 词：噬菌体裂解酶 小球藻 电转化 抗菌药物 

分 类 号：Q939.48[生物学—微生物学]