Ⅱ型糖尿病模型小鼠粪便微生物多样性分析  

Analysis of fecal microbial diversity in a typeⅡdiabetes mouse model

作　　者：尚小龙 朱珠 张倍明 林金杏 易兰兰 张淑珍 李智鹏 魏晓峰 谢淑武 范春 赵素梅[4] 赵生国[1] 赵彦光 SHANG Xiaolong;ZHU Zhu;ZHANG Beiming;LIN Jinxing;YI Lanlan;ZHNG Shuzhen;LI Zhipeng;WEI Xiaofeng;XIE Shuwu;FAN Chun;ZHAO Sumei;ZHAO Shengguo;ZHAO Yanguang(College of Animal Science and Technology,Gansu Agricultural University,Lanzhou 730070,China;Shanghai Laboratory Animal Research Center,Shanghai 201201;Science Island Branch,Graduate School of University of Science and Technology of China,Hefei 230026,China;College of Animal Science and Technology,Yunnan Agricultural University,Kunming 650201,China)

机构地区：[1]甘肃农业大学动物科学技术学院,甘肃兰州730070 [2]上海实验动物研究中心,上海201201 [3]中国科学技术大学研究生院科学岛分院,安徽合肥230026 [4]云南农业大学动物科学技术学院,云南昆明650201

出　　处：《甘肃农业大学学报》2024年第2期24-35,共12页Journal of Gansu Agricultural University

基　　金：州校合作项目(2020ZXND02);科技创新人才计划(2020FA011)。

摘　　要：【目的】揭示粪便微生物对Ⅱ型糖尿病的作用以及影响。【方法】以SPF级BKS-DB雄性小鼠为T2DM模型,通过测定对照组(CN组)和模型组(T2DM组)粪便微生物16SrRNA基因分析其群落结构与组成。【结果】门水平上,发现T2DM模型组小鼠粪便中蝴蝶菌门、弯曲杆菌门菌群丰度显著高于CN组。属水平中,T2DM组发现16个特有菌属,其中丰度占比最大的是库特氏菌属。在差异显著的前10个菌属中,9个菌属T2DM组菌群丰度显著高于CN组(P<0.05),仅有1个菌群丰度显著低于CN组。通过LEfSe分析,发现厌氧菌属、棒状杆菌属在T2DM模型组出现富集,而这两种菌属都存在于免疫力低下的人群中,与糖尿病呈正相关存在。【结论】在T2DM模型小鼠的粪便中,蝴蝶菌门、拟杆菌属等菌群出现高表达现象,同时,厌氧菌属、棒状杆菌属也出现了失衡,这可能导致了机体免疫力降低,正向影响糖尿病的发生。【Objective】To elucidate the role and influence of fecal microorganisms on typeⅡdiabetes mellitus(T2DM).【Method】In this study,SPF-grade BKS-DB male mice served as models for T2DM.The community structure and composition of fecal microbial 16S rRNA genes were analyzed in both the control group(CN group)and the T2DM model group(T2DM group).【Result】At the phylum level,the abundance of Patescibacteria and Campilobacterota in the feces of mice in the T2DM group was significantly higher than that in the CN group.At the genus level,16 endemic genera were identified in the T2DM group,with Kurthia being the most abundant.Among the top 10 genera with significant differences,the abundance of flora in 9 genera in the T2DM group was significantly higher than in the CN group(P<0.05),while the abundance of microbiota in 1 genus in the T2DM group was significantly lower.LEfSe analysis revealed that the genera Anaerofustis and Corynebacterium were enriched in the T2DM group and are both associated with immunocompromised individuals,showing a positive correlation with diabetes.【Conclusion】In the feces of T2DM model mice,high expression of flora such as Patescibacteria and Bacteroidota was observed,along with an imbalance between the genera Anaerofustis and Corynebacterium.This imbalance may contribute to decreased immune function and positively influence the development of diabetes.

关 键 词：Ⅱ型糖尿病 Ⅱ型糖尿病模型小鼠 粪便微生物 

分 类 号：R587.1[医药卫生—内分泌]