转录组和蛋白质组关联分析解析巴西蕉幼苗响应低温的分子机制  

Transcriptome and Proteome Association Analysis to Revealthe Molecular Mechanism of Baxi Banana Seedlings in Response to Low Temperature

作　　者：林蔚 吴水金 李跃森 LIN Wei;WU ShuiJin;LI YueSen(Subtropical Agriculture Research Institute,Fujian Academy of Agricultural Sciences,Zhangzhou 363005,Fujian)

机构地区：[1]福建省农业科学院亚热带农业研究所,福建漳州363005

出　　处：《中国农业科学》2024年第8期1575-1591,共17页Scientia Agricultura Sinica

基　　金：福建省公益类科研院所专项(2022R1030006,2021R1030006);福建省自然科学基金(2023J01374)。

摘　　要：[目的]低温是导致香蕉减产的主要自然灾害之一。基于转录组与蛋白质组关联分析参与香蕉抗寒的相关基因、蛋白及信号、代谢通路调控网络,探讨香蕉抗寒的分子机制。[方法]以巴西蕉为试材,在7℃低温下处理1 d(Cold1)和3 d(Cold3),以28℃培养的巴西蕉为对照(CK),基于笔者课题组前期获得的蛋白质组数据,利用转录组测序技术检测巴西蕉在低温胁迫下基因调控网络的变化,同时与蛋白质组学数据进行关联分析,共同解析巴西蕉响应低温胁迫的分子机制。[结果]转录组分析结果显示,Cold1 vs CK、Cold3 vs CK和Cold1 vs Cold3三个对比组分别鉴定出11 370、15 460和9 619个差异表达基因,对这些基因的KEGG富集分析发现,差异表达基因在光合作用信号、谷胱甘肽代谢、α-亚麻酸代谢途径和苯丙素生物合成等多个低温胁迫关键信号代谢通路中富集。对部分差异表达基因进行实时荧光定量(qRT-PCR)分析,其中DREB、MAPK和MYB等低温调控关键基因的表达量在低温处理后显著上升,所选20个基因的表达变化趋势与RNA-seq基本相符,证实了RNA-seq的准确性。转录组与蛋白质组关联分析结果显示,共鉴定到6 211个与转录本相对应的蛋白,转录组与蛋白质呈现正相关关系,共有105个转录本及其对应的蛋白表达量共同上调,有218个转录本及其对应的蛋白表达量共同下调。GO富集分析显示,差异表达基因和蛋白在光响应、叶绿体、氧化还原酶活性等功能大量富集。此外,对差异表达基因和蛋白KEGG通路的关联分析发现,低温处理抑制了苯丙素生物合成和光合作用信号途径相关基因及蛋白的表达,促进了α-亚麻酸代谢和谷胱甘肽途径的表达。[结论]运用转录组结合蛋白质组学技术绘制了基因和蛋白质水平上的香蕉抗寒调控网络,并发现香蕉响应低温的信号通路主要涉及光合作用信号、谷胱甘肽代谢、α-亚麻酸代谢[Objective]Low temperature is a significant natural disaster that affects banana production.In this study,based on transcriptome and proteome association analysis,the regulatory network of genes,proteins,signals and metabolic pathways involved in banana cold resistance was investigated.The aim was to explore the molecular mechanism of banana cold resistance.[Methods]‘Baxi’banana(Musa nana Lour)was treated at 7℃ for 1 and 3 d,and a control group was treated at 28℃.Based on the proteome data obtained in the previous study,the transcriptome sequencing technology was used to detect changes in the gene regulatory network of banana under cold stress.Simultaneously,the correlation analysis was conducted with proteomics to analyze the molecular mechanism of banana response to cold stress.[Result]Transcriptome analysis revealed that 11370,15460 and 9619 differentially expressed genes were identified in the three comparison groups of Cold1 vs CK,Cold3 vs CK and Cold1 vs Cold3,respectively.KEGG enrichment analysis of these genes revealed that the differentially expressed genes were enriched in several key signaling metabolic pathways,such as photosynthesis signal,glutathione metabolism,α-linolenic acid metabolic pathway and phenylpropanoid biosynthesis under low temperature stress.Moreover,there were significant differences in the enrichment degree of glutathione metabolic pathway between Cold1 d vs CK and Cold3 d vs CK.Real-time quantitative PCR analysis(qRT-PCR)was performed on several differentially expressed genes.Among them,the expression levels of key low-temperature regulatory genes,such as DREB,MAPK and MYB,were significantly increased after the low-temperature treatment.The expression trend of the selected 20 genes was essentially consistent with that of RNA-seq,confirming the accuracy of RNA-seq.The results of the transcriptome and proteome association analysis showed a positive correlation between the transcriptome and proteomics.A total of 6211 proteins corresponding to transcripts were identified.Among

关 键 词：香蕉 低温胁迫 转录组 蛋白质组 关联分析 

分 类 号：S668.1[农业科学—果树学]