中华鲟IFNe2的抗病毒天然免疫作用  

Antiviral innate immune function of Chinese sturgeon(Acipenser sinensis)IFNe2

作　　者：李由申 丁广义 张书环 郭慧芝 周江 罗凯[1] 许巧情[1] LI Youshen;DING Guangyi;ZHANG Shuhuan;GUO Huizhi;ZHOU Jiang;LUO Kai;XU Qiaoqing(Institute of Chinese Sturgeon Disease,Yangtze University,Jingzhou 434020,China;Sturgeon Healthy Breeding and Medicinal Value Research Center,Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China)

机构地区：[1]长江大学中华鲟病害研究所,湖北荆州434020 [2]贵州中医药大学,鲟鱼健康养殖与药用价值研究中心,贵州贵阳550025

出　　处：《水产学报》2024年第5期44-53,共10页Journal of Fisheries of China

基　　金：国家自然科学基金(32260925);贵州省科技厅项(黔-ZK[2022]507)。

摘　　要：为探究中华鲟干扰素e2(AsIFNe2)的免疫调控作用,本实验通过原核表达获得了重组中华鲟干扰素e2蛋白(rAsIFNe2),并分析其对抗病毒相关基因的影响及其抗病毒活性。实时荧光定量PCR(RT-qPCR)结果显示,rAsIFNe2能显著激活中华鲟鳍细胞中干扰素刺激基因(IFN-stimulated genes,ISGs)的表达,如Mx、PKR、Viperin和ADAR4。此外,rAsIFNe2还能通过激活IRFs和IFNes基因的表达,从而帮助宿主细胞迅速建立抗病毒状态。在鲤春病毒血症病毒(SVCV)感染鲤上皮细胞(EPC)模型中,rAsIFNe2能够诱导EPC细胞中Mx、PKR和Viperin的表达,并降低EPC细胞中SVCV病毒G、N和P基因的表达,减少病变效应的产生。研究表明,AsIFNe2在宿主抗病毒天然免疫反应中发挥作用。本研究为深入了解中华鲟的干扰素免疫系统以及治疗病毒性疾病提供理论依据。Chinese sturgeon(Acipenser sinensis)is a critically endangered fish species found in the Yangtze River of China,for which there is scarce immunological research.TypeⅠinterferons(IFNs)are widely recognized as pivotal cytokines in the host's antiviral immune response.Therefore,the present study aimed to investigate the immune function of interferon e2 in A.sinensis(rAsIFNe2).The recombinant A.sinensis interferon e2 protein(rAsIFNe2)was expressed through prokaryotic expression,and its effects on antiviral-related genes and its antiviral activity were analyzed.Real-time quantitative PCR(RT-qPCR)results showed that rAsIFNe2 significantly activated the expression of interferon-stimulated genes(ISGs)in A.sinensis fin cells,such as Mx,Viperin,PKR,and ADAR4 with 460.95-fold and 669.01-fold changes at 24 h,a 42.76-fold change at 6 h,and 6.72 fold change at 12 h of incubation,respectively.Furthermore,rAsIFNe2 also helped host cells establish an antiviral state by activating the expression of IRF1,IRF2,IRF3,IRF7,IFNe1,IFNe2 and IFNe3 genes with increases by 11.56,3.08,9.72 and 1083.07 times at 6 h,a 2.15-fold change at 3 h,a 119.15-fold change at 12 h,and a-6.88 fold change at 3 h of incubation,respectively.In the spring viremia of carp virus(SVCV)-infected carp epithelial cells(EPC)model,rAsIFNe2 induced the expression of Mx,PKR,and Viperin in EPC cells with 13.29-fold and 14.36-fold changes at 6 h,and a 19.25-fold change at 24 h of infection.The SVCV virus G,N,and P genes in EPC cells were significantly downregulated by rAsIFNe2 with 388.50,259.74 and 979.91-fold changes after 24 h,thereby reducing the lesions.These results indicate that AsIFNe2 plays a role in the host's antiviral innate immune response,providing a theoretical basis for understanding the interferon immune system of A.sinensis and treating viral diseases.

关 键 词：中华鲟 干扰素 抗病毒 鲤春病毒血症病毒 免疫调控 

分 类 号：S942.1[农业科学—水产养殖]