水稻OsMGD2和OsMGD3基因的功能鉴定及其对烟草耐低磷胁迫的影响  

Functional identification of the OsMGD2 and OsMGD3 genes in rice and their influence on phosphorus deficiency in tobacco

作　　者：席源 张梅娟 李莎莎[1] 王灵龙 殷俐娜[1,4] 王仕稳[1,4] XI Yuan;ZHANG Meijuan;LI Shasha;WANG Linglong;YIN Li'na;WANG Shiwen(College of Resources and Environment,Northwest A&F University,Yangling,Shaanxi 712100,China;School of Biomedical and Food Engineering,Shangluo University,Shangluo,Shaanxi 726000,China;College of Agricultural,Northwest A&F University,Yangling,Shaanxi 712100,China;State Key Laboratory of Soil Erosion and Dryland Farming on the loess Plateau,Institute of Soil and Water Conservation,Chinese Academy of Sciences,Ministry of Water Resources,Yangling,Shaanxi 712100,China)

机构地区：[1]西北农林科技大学资源环境学院,陕西杨凌712100 [2]商洛学院生物医药与食品工程学院,陕西商洛726000 [3]西北农林科技大学农学院,陕西杨凌712100 [4]中国科学院水利部水土保持研究所,黄土高原土壤侵蚀与旱地农业国家重点实验室,陕西杨凌712100

出　　处：《西北植物学报》2024年第5期716-728,共13页Acta Botanica Boreali-Occidentalia Sinica

基　　金：陕西省自然科学基础研究计划项目(2021JM-604)。

摘　　要：【目的】单半乳糖甘油二酯合酶(MGD)是合成单半乳糖甘油二酯(MGDG)的关键酶,在植物响应低磷胁迫过程中起着重要作用。为系统了解水稻OsMGD2和OsMGD3基因在低磷胁迫响应中的作用和功能。【方法】用盆栽试验分析正常和低磷条件下野生型(SR1)、过表达OsMGD2和OsMGD3转基因烟草的生理响应及脂质组分的变化。【结果】无论在正常还是低磷条件下,转基因与野生型烟草的磷含量无显著差异,但转基因烟草的生物量、叶绿素含量和光合能力均显著高于野生型。转基因烟草在低磷胁迫下的磷脂(PL)含量、双半乳糖甘油二酯(DGDG)含量、DGDG与MGDG的比值和半乳糖脂(GL)与PL的比值均显著高于野生型烟草,且OsMGD3转基因烟草的脂质含量和比值均高于OsMGD2转基因烟草。【结论】通过调控水稻OsMGD2/3基因表达,可提高植物低磷下的膜脂重塑能力,进而维持植物在低磷胁迫下较高的光合和生长能力,增加植物的低磷耐受性。[Objective]Monogalactose diglyceride synthase(MGD)is a key enzyme in the synthesis of monogalactose diglyceride(MGDG)and plays an important role in plant response to low phosphorus tolerance.We aim to understand the role of the OsMGD2 and OsMGD3 genes of rice in response to phosphorus(P)deficiency.[Methods]A pot experiment was conducted to analyze physiological responses and lipid composition changes of the wild-type(SR1)and transgenic tobacco under normal and low P condition.[Results]There was no difference in P content between the transgenic and wild-type tobacco under normal and P deficiency conditions.However,the biomass,chlorophyll content,and photosynthetic electronic transfer of the transgenic tobacco were higher than wild-type.The phospholipid(PL)content,digalactose diglyceride(DGDG)content,DGDG/MGDG ratio,and GL/PL ratio of the transgenic tobacco were higher than those of the wild-type tobacco under low phosphorus deficiency,and the lipid content and ratio of the tobacco expressing OsMGD3 were higher than the tobacco expressing OsMGD2.[Conclusion]Regulation of OsMGD2/3 gene expression in rice can improve lipid remodeling ability and maintain higher photosynthetic and growth capacity under phosphorus deficiency,increasing plant tolerance to low phosphorus stress.

关 键 词：水稻 低磷胁迫 转基因烟草 膜脂重塑 单半乳糖甘油二酯合酶 单半乳糖甘油二酯 双半乳糖甘油二酯 磷脂 

分 类 号：Q945.78[生物学—植物学] S511[农业科学—作物学]