荔枝VQ基因家族鉴定及其对非生物胁迫的响应  

Identification of the VQ gene family and their responses to abiotic stresses in Litchi chinensis

作　　者：凡超[1] 杨杰 陈蓉 刘伟[1] 向旭[1] FAN Chao;YANG Jie;CHEN Rong;LIU Wei;XIANG Xu(Institute of Fruit Tree Research,Guangdong Academy of Agricultural Sciences,Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization,Ministry of Agriculture and Rural Affairs,Guangdong Provincial Key Laboratory of Tropical and Subtropical Fruit Tree Research,Guangzhou 510640,China)

机构地区：[1]广东省农业科学院果树研究所,农业农村部南亚热带果树生物学与遗传资源利用重点实验室,广东省热带亚热带果树研究重点实验室,广州510640

出　　处：《西北植物学报》2024年第5期739-750,共12页Acta Botanica Boreali-Occidentalia Sinica

基　　金：乡村振兴战略专项资金(农业科技能力提升)项目(2023TS-2-3);茂名市科技计划项目(2022DZXHT058);汕尾市科技计划项目(2022A003)。

摘　　要：【目的】VQ蛋白是一类含有保守VQ基序(FxxhVQxhTG)的植物特异性蛋白,在植物生长发育和非生物胁迫应答中发挥重要作用。研究鉴定了荔枝VQ基因家族,并分析其在不同组织的表达模式及在低温、高温、干旱和盐胁迫下的应答,为后续研究其抗逆机制奠定了基础。【方法】用生物信息学方法在荔枝全基因组中鉴定LcVQ基因,并对其理化性质、亚细胞定位、基因结构和保守基序等进行分析;用MEGA 6.0软件构建系统发育树,分析荔枝、拟南芥和水稻VQ蛋白的系统发育关系;用实时荧光定量PCR(qRT-PCR)技术验证LcVQs对多种非生物胁迫的响应情况。【结果】荔枝中共鉴定获得可聚类为9个亚族的18个VQ基因(LcVQ1-18),依次分布在荔枝的11条染色体上,其编码蛋白的氨基酸数介于111~427之间,分子质量为12.48~45.49 kD;除LcVQ15和LcVQ17定位于细胞质之外,其余LcVQ蛋白均定位于细胞核。LcVQs启动子区域包含大量植物生长发育响应元件、激素响应元件及逆境响应元件。LcVQs的表达量在不同组织中具有明显差异,总体上分为普遍性表达和特异性表达。LcVQs可快速响应非生物胁迫,在低温、高温、干旱和盐胁迫处理3 h内分别有4,3,3,4个LcVQs明显上调表达。【结论】荔枝全基因组中有18个VQ家族成员,具有典型VQ保守结构域,能差异化响应多种非生物胁迫。[Objective]VQ proteins are plant-specific proteins with the conserved VQ motifs(Fxxh-VQxhTG),which play an important role in plant growth and development as well as in response to abiotic stresses.The role of the VQ gene family in litchi(Litchi chinensis)had not been systematically studied.We identified the LcVQ gene family members as well as their expression patterns in different tissues of lychee and their responses to abiotic stresses.This laid a foundation for studying its stress-resistant mechanism.[Methods]The sequences of LcVQ genes were obtained from the L.chinensis genome using bioinformatics methods,and their physicochemical properties,subcellular localizations,gene structures,and conserved motifs were analyzed;a phylogenetic tree was constructed using MEGA 6.0 to analyze the phylogenetic relationships of the VQ proteins in L.chinensis,Arabidopsis,and Oryza sativa;the responses to various abiotic stresses were verified by the quantitative real-time PCR(qRT-PCR).[Results]A total of 18 LcVQ genes were identified from L.chinensis genome,named as LcVQ1-18,which were clustered into 9 subgroups.They were widely distributed on 11 of 15 chromosomes of lychee.The amino acid numbers of LcVQ proteins were ranged from 111 to 427,and molecular weights from 12.48 kD to 45.49 kD.Subcellular localizations showed that except for LcVQ15 and LcVQ17 were localized at the cytoplasm,all other LcVQ proteins were localized at the nucleus.The LcVQ promoters contained a large number of growth and development,plant hormone,and stress-responsiveness related response elements.The LcVQ genes expression was varied at different tissues,which was divided into universal expression and specific expression.Under the treatment of low temperature,high temperature,drought,and salt stress,we found that 4,3,3,and 4 LcVQ genes were significantly up-regulated at 3 hours of treatment,respectively,indicating that these genes could respond to the abiotic stresses.[Conclusion]There are 18 VQ members in the L.chinensis genome with typical VQ binding

关 键 词：荔枝 VQ基因家族 生物信息学 非生物胁迫 表达分析 

分 类 号：Q943.2[生物学—植物学] Q786[农业科学—果树学] S667.1[农业科学—园艺学]