陆地棉SAURX克隆及表达模式分析  

Cloning and expression analysis of the SAURX gene in Gossypium hirsutum

作　　者：刘永昌 马晓梅[2] 吴忠新 孙玉进 陈纳 LIU Yongchang;MA Xiaomei;WU Zhongxin;SUN Yujin;CHEN Na(College of Bioengineering,Jingchu University of Technology,Jingmen,Hubei 448000,China;Cotton Research Institute,Xinjiang Science Academy of Agriculture and Reclaimation,Shihezi,Xinjiang 832000,China)

机构地区：[1]荆楚理工学院生物工程学院,湖北荆门448000 [2]新疆农垦科学院棉花研究所,新疆石河子832000

出　　处：《西北植物学报》2024年第5期751-759,共9页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金项目(31960439);荆楚理工学院科学研究项目(ZD202301);特色花卉生物育种湖北省工程中心重大项目(2023TD004)。

摘　　要：【目的】生长素上调的小RNA(SAURs)是一类生长素原初响应基因,在细胞伸长的过程中具有重要作用。克隆纤维发育相关的SAUR基因,研究其表达模式和生物学功能对探究纤维发育的机理具有重要意义。【方法】从陆地棉中克隆到1个编码SAUR蛋白的基因,命名为GhSAURX,通过表达模式分析、亚细胞定位、转基因拟南芥表型分析及生长素相关基因表达分析初步研究其功能。【结果】进化树分析表明:GhSAURX与拟南芥SAUR76、SAUR77和SAUR78关系较近,属于SAUR76亚家族。qPCR结果发现,GhSAURX基因在快速伸长的纤维中表达量较高,即开花后15,18,21 d。同时,GhSAURX在长纤维品种中的表达水平明显高于短纤维。亚细胞定位分析显示,GhSAURX定位在细胞膜和细胞核中。异源表达GhSAURX可以提高YUCCA6、ARF7及PIN4的表达,增加拟南芥的主根长度和黑暗条件下胚轴的长度。【结论】GhSAURX可能在生长素调控细胞伸长的过程中具有重要作用。[Objective]Small auxin up RNAs(SAURs),which belong to a kind of auxin early response gene,play an key role in plant cell elongation.This study aims to clone fiber development-related SAUR genes,study their expression pattern and biological function.There is of great significance for exploring the mechanism of fiber development.[Methods]A gene encoding SAUR protein was cloned from Gossypium hirsutum,and was named GhSAURX.Expression pattern,subcellular localization,phenotypic identification of the transgenic Arabidopsis,and expression of auxin-related genes were performed.[Results]Phylogenetic analysis revealed that GhSAURX had close relationship with SAUR76,SAUR77,and SAUR78 in Arabidopsis,and was belonged to the SAUR76 subfamily.qRT-PCR showed that the highest expression level of GhSAURX was observed in fiber of 15,18 and 21 DPA,when fiber was elongating fast.Meanwhile,GhSAURX showed much higher expression level in varieties with long fiber than that in varieties with short fiber.Analysis of subcellular localization indicated that GhSAURX was distributed in cytoplasm and nucleus.Overexpression of GhSAURX promoted the elongation of the primary roots and hypocotyls under dark condition in Arabidopsis,and enhanced the expression of auxin-related genes,such as YUCCA6,ARF7,and PIN4.[Conclusion]Our results indicated that GhSAURX functions in the cell elongation mediated by auxin.

关 键 词：陆地棉 生长素 SAUR 细胞伸长 表达分析 

分 类 号：Q785[生物学—分子生物学] Q786[农业科学—作物学] S562