拟杆菌对高能低蛋白饲粮诱导的脂肪肝出血综合征蛋鸡肝脏脂质代谢的影响  

Effect of Bacteroides on liver lipid metabolism of laying hens with fatty liver hemorrhage syndrome induced by high energy and low protein diet

作　　者：刘海隆 张艳 马榕菲 张雨 赵兴华[2] 何欣[2] LIU Hai-long;ZHANG Yan;MA Rong-fei;ZHANG Yu;ZHAO Xing-hua;HE Xin

机构地区：[1]海南省农业科学院畜牧兽医研究所,海南海口571100 [2]河北农业大学动物医学院,河北保定071001

出　　处：《饲料研究》2024年第7期39-43,共5页Feed Research

基　　金：海南省属科研院所技术创新专项(项目编号:SQKY2022-0006)。

摘　　要：研究旨在探讨拟杆菌对高能低蛋白饲粮诱导的脂肪肝出血综合征(FLHS)蛋鸡肝脏脂质代谢的影响。选取80只210日龄体重为(1.5±0.2)kg的大午金凤蛋鸡,随机分为4组,每组5个重复,每个重复4只鸡。对照组(C组)饲喂基础饲粮,模型组(M组)饲喂高能低蛋白(HELP)饲粮,拟杆菌低剂量组(BL组)饲喂HELP饲粮+1×10^(9)CFU/mL拟杆菌,拟杆菌高量组(BH组)饲喂HELP饲粮+1×10^(11)CFU/mL拟杆菌。试验期70 d。结果显示:与C组相比,M组蛋鸡肝脏油腻、质脆易碎,出现大量脂肪空泡和红染脂滴;肝脏指数、腹脂率显著上升(P<0.05);血清总甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)含量及谷草转氨酶(AST)、谷丙转氨酶(ALT)活性显著上升(P<0.05),高密度脂蛋白胆固醇(HDL-C)含量显著下降(P<0.05)。与M组相比,BL组和BH组蛋鸡肝脏脂肪空泡和红染脂滴有明显改善;肝脏指数显著降低(P<0.05);血清TG、TC、LDL-C含量及AST、ALT活性均显著下降(P<0.05),HDL-C含量显著上升(P<0.05)。与BH组相比,BL组蛋鸡血清TG、TC含量及AST活性显著降低(P<0.05),HDL-C含量显著升高(P<0.05)。研究表明,拟杆菌可改善FLHS引起的蛋鸡脂质代谢紊乱和肝功能损伤,添加1×10^(9)CFU/mL拟杆菌对调节FLHS蛋鸡的肝脏脂质代谢效果更佳。The purpose of the study was to investigate the effect of Bacteroides on liver lipid metabolism in laying hens with fatty liver hemorrhage syndrome(FLHS)induced by high-energy and low-protein diet.Eighty 210-day-old Dawu Jinfeng laying hens weighing(1.5±0.2)kg were randomly divided into four groups with five replicates in each group and four hens in each replicate.The control group(Group C)was fed with basal diet,the model group(Group M)was fed with high energy and low protein(HELP)diet,the low dose Bacteroides group(Group BL)was fed with HELP diet+1×10^(9)CFU/mL Bacteroides,and the high dose Bacteroides group(Group BH)was fed with HELP diet+1×10^(11)CFU/mL Bacteroides.The trial period was 70 days.The results showed that compared with Group C,the liver of Group M was greasy,brittle and fragile,with a large number of fat vacuoles and red-stained fat droplets,the liver index and abdominal fat rate increased significantly(P<0.05),and the serum TG,TC,LDL-C concentrations and AST,ALT activities increased significantly(P<0.05),while HDL-C concentration decreased significantly(P<0.05).Compared with Group M,Group BL and Group BH showed significant improvement in liver fat vacuoles and red stained lipid droplets in laying hens,the liver index significantly decreased(P<0.05),the levels of serum TG,TC,LDL-C,as well as the activities of AST and ALT,were significantly reduced(P<0.05),while the levels of HDL-C were significantly increased(P<0.05).Compared with Group BH,serum TG,TC concentrations and AST activity in BL group decreased significantly(P<0.05),while HDL-C concentration increased significantly(P<0.05).Study shows that Bacteroides can improve lipid metabolism disorder and liver function damage caused by FLHS.Bacteroides with 1×10^(9)CFU/mL is more effective in regulating liver lipid metabolism in FLHS laying hens.

关 键 词：拟杆菌 蛋鸡 脂肪肝出血综合征 脂质代谢 

分 类 号：S831[农业科学—畜牧学]