沙枣叶水提取物的体外抗菌活性机制研究  

In vitro antibacterial activity mechanism of Elaeagnus angustifolia L.leaves water extract

作　　者：唐莎莎 宫海燕 于浩楠 孙芸[2] TANG Sha-sha;GONG Hai-yan;YU Hao-nan;SUN Yun

机构地区：[1]内江卫生与健康职业学院,四川内江641100 [2]新疆医科大学中医学院,新疆乌鲁木齐830000 [3]广东医科大学附属东莞第一医院,广东东莞523000

出　　处：《饲料研究》2024年第7期88-93,共6页Feed Research

基　　金：国家自然科学基金项目(项目编号:81860772)。

摘　　要：试验旨在测定沙枣叶水提取物对易引起动物感染的致病菌的体外抗菌活性,揭示沙枣叶水提取物抗菌机制。采用96孔板微量稀释法初步筛选沙枣叶水提取物的抗菌谱,绘制致病菌生长曲线,测定碱性磷酸酶活性、蛋白质质量浓度,结合透射电子显微镜综合分析沙枣叶水提取物对主要致病菌金黄色葡萄球菌的抑制作用机制。结果显示,沙枣叶水提取物对金黄色葡萄球菌、表皮葡萄球菌、粪肠球菌、铜绿假单胞菌、大肠杆菌、阴沟肠杆菌、白色念珠菌均具有不同程度的抑制作用,能够破坏致病菌细胞壁、细胞膜的通透性和完整性,抑制其生长、代谢。研究表明,沙枣叶水提取物能够抑制常见的金黄色葡萄球菌等致病菌的生长和代谢,具有较强的抑菌功能。The purpose of this experiment was to determine the antibacterial activity of Elaeagnus angustifolia L.leaves water extract against pathogenic bacteria easily causing animal infection in vitro,and to reveal the antibacterial mechanism of Elaeagnus angustifolia L.leaves water extract.The antibacterial spectrum of Elaeagnus angustifolia L.leaves water extract was screened by a 96-well plate microdilution method.The growth curve of pathogenic bacteria was drawn,the activity of alkaline phosphatase and protein mass concentration were determined,and the inhibitory mechanism of Elaeagnus angustifolia L.leaves water extract against Staphylococcus aureus was comprehensively analyzed by transmission electron microscope.The results showed that the water extract of Elaeagnus angustifolia L.leaves had different inhibitory effects on Staphylococcus aureus,Staphylococcus epidermidis,Enterococcus faecalis,Pseudomonas aeruginosa,Escherichia coli,Enterobacter cloacae,and Candida albicans,which could destroy the permeability and integrity of cell wall and membrane of pathogenic bacteria and inhibit their growth and metabolism.Studies has shown that the Elaeagnus angustifolia L.leaves water extract can inhibit the growth and metabolism of common Staphylococcus aureus and other pathogenic bacteria,and has a strong antibacterial function.

关 键 词：沙枣叶 饲料添加剂 金黄色葡萄球菌 抑菌机制 

分 类 号：S816.7[农业科学—饲料科学]