手足口病患儿外周血TLR3、s100β蛋白、IL-6的表达水平及其与病情和预后的相关性  

Expression levels of TLR3,s100βprotein,and IL-6 in peripheral blood of children with hand-foot-and-mouth disease and their correlation with disease condition and prognosis

作　　者：冯晓靖 袁艳[1] 王芳[1] FENG Xiao-jing;YUAN Yan;WANG Fang(Department of Infectious Diseases,Children's Hospital Affiliated to Zhengzhou University/Henan Children's Hospital/Zhengzhou Children's Hospital,Zhengzhou 450000,Henan,CHINA)

机构地区：[1]郑州大学附属儿童医院、河南省儿童医院、郑州儿童医院感染性疾病科,河南郑州450000

出　　处：《海南医学》2024年第10期1446-1451,共6页Hainan Medical Journal

基　　金：2022年度河南省医学科技攻关计划联合共建项目(编号:LHGJ20220753)。

摘　　要：目的检测手足口病(HFMD)患儿外周血Toll样受体3(TLR3)、中枢神经特异性蛋白100β(S100β)和白细胞介素-6(IL-6)的表达水平,并探讨其与患儿病情和预后的关系。方法选择2019年1月至2023年1月郑州大学附属儿童医院、河南省儿童医院、郑州儿童医院收治的142例HFMD患儿作为观察组,依据患儿病情严重程度(轻症组与重症组)、年龄(低龄组与高龄组)和预后(良好组与不良组)进行分组,另选取87例同期健康儿童作为对照组。比较不同病情严重程度患儿与健康儿童的外周血TLR3、s100β、IL-6水平,比较观察组患儿入院不同时间、不同病情严重程度患儿入院不同时间、不同年龄患儿入院不同时间以及不同预后患儿入院不同时间的外周血TLR3、s100β、IL-6水平。结果重症组患儿的外周血TLR3、s100β、IL-6表达水平分别为1.20±0.36、(14.41±3.53)μg/L、(14.98±4.37)U/m,明显高于轻症组的0.84±0.31、(3.12±1.01)μg/L、(10.73±3.05)U/m和对照组的0.68±0.19、(1.31±0.17)μg/L、(8.95±2.36)U/m,轻症患儿的外周血TLR3、s100β、IL-6表达水平明显高于对照组,差异均有统计学意义(P<0.05);观察组患儿入院1 d的外周血TLR3、s100β、IL-6表达水平明显低于入院5 d、7 d,入院7 d的外周血TLR3、s100β、IL-6表达水平明显低于入院5d时,差异均有统计学意义(P<0.05);重症组患儿入院1d、5d、7d的外周血TLR3、s100β、IL-6表达水平明显高于轻症组,差异均有统计学意义(P<0.05);低龄组患儿入院1 d、5 d、7 d的外周血TLR3、s100β、IL-6表达水平明显高于高龄组,差异均有统计学意义(P<0.05);预后良好组患儿入院1 d、7 d的外周血TLR3表达水平分别为0.97±0.31、1.90±0.55,明显低于预后不良组的1.90±0.18、2.46±0.74,入院1 d、5 d、7 d的外周血s100β、IL-6表达水平分别为(8.17±2.31)μg/L、(32.98±10.08)μg/L、(27.27±7.66)μg/L、(12.65±4.09)U/mL、(17.41±4.06)U/mL、(14.39±4.35)U/mL,明显低于预后Objective To detect the expression levels of peripheral blood Toll-like receptor 3(TLR3),central nerve specific protein 100β(S100β),and interleukin-6(IL-6)in children with hand-foot-and-mouth disease(HFMD)and to explore their relationship with disease condition and prognosis of children.Methods A total of 142 children with HFMD admitted to Children's Hospital Affiliated to Zhengzhou University/Henan Children's Hospital/Zhengzhou Children's Hospital from January 2019 to January 2023 were selected as observation group and were grouped according to the severity of disease(mild group and severe group),age(young group and older group),and prognosis(good prog-nosis group and poor prognosis group).Eighty-seven healthy children were enrolled as control group.The levels of TLR3,s100β,and IL-6 in peripheral blood of children with different disease severities and healthy children were com-pared,and the levels of peripheral blood TLR3,s100β,and IL-6 were compared in the observation group at different time points of admission,among patients with different disease severities,different ages,and different prognosis status at different time points of admission.Results The expression levels of TLR3,s100β,and IL-6 in peripheral blood of children in the severe group were 1.20±0.36,(14.41±3.53)μg/L,and(14.98±4.37)U/m,significantly higher than 0.84±0.31,(3.12±1.01)μg/L,and(10.73±3.05)U/m in the mild group and 0.68±0.19,(1.31±0.17)μg/L,and(8.95±2.36)U/m in the control group;the above levels in the mild group were significantly higher than those in the control group;the differences were statistically significant(P<0.05).The expression levels of peripheral blood TLR3,s100β,and IL-6 in the observation group at 1 day after admission were significantly lower than those at 5 and 7 days after admission,and the expression lev-els at 7 days after admission were significantly lower than those at 5 days after admission,with statistically significant dif-ferences(P<0.05).The expression levels of TLR3,s100β,and IL-6 in peripheral

关 键 词：手足口病 外周血Toll样受体3 中枢神经特异性蛋白100β 白细胞介素-6 病情 预后 相关性 

分 类 号：R729[医药卫生—儿科]