4-丁基间苯二酚微乳凝胶制备及美白抗氧化研究  

Preparation of 4-butylresorcinol-loaded cinnamon oil microemulsion-gel and its whitening and antioxidative effects

作　　者：魏楚原 张晓萍 潘敬 彭佩 张雅鹃 穆朝峰 Chuyuan Wei;Xiaoping Zhang;Jing Pan;Pei Peng;Yajuan Zhang;Chaofeng Mu(School of Pharmaceutical Sciences,Zhejiang Chinese Medical University,Hangzhou,Zhejiang 311402,China)

机构地区：[1]浙江中医药大学药学院,浙江杭州311402

出　　处：《日用化学工业（中英文）》2024年第5期520-526,共7页China Surfactant Detergent & Cosmetics

基　　金：国家级大学生创新创业训练计划项目(202210344058X)。

摘　　要：通过配方筛选优化制备含有4-丁基间苯二酚的肉桂油微乳凝胶,考察其制剂学性质,并对其进行美白及抗氧化性能的评价。含有4-丁基间苯二酚的肉桂油微乳外观澄清透明,粒径15.68 nm、多分散系数(PDI)0.185。以卡波姆和白芨多糖作为凝胶基质制备微乳凝胶,该微乳凝胶的pH值为6.0,黏度为74.8 Pa·s,符合外用凝胶剂质量要求。通过酪氨酸酶抑制率实验测定4-丁基间苯二酚肉桂油微乳凝胶的美白功效,该微乳凝胶的酪氨酸酶抑制率随着质量浓度增加而逐渐增强,且微乳凝胶的酪氨酸酶抑制率均高于同等质量浓度下的游离4-丁基间苯二酚和熊果苷,表明4-丁基间苯二酚肉桂油微乳凝胶具有良好的美白功效。通过4-丁基间苯二酚肉桂油微乳凝胶对过氧化氢(H_(2)O_(2))诱导B16-F10细胞氧化损伤保护作用的测定,评价其抗氧化性能,结果表明4-丁基间苯二酚肉桂油微乳凝胶在0.1μg/mL质量浓度下无细胞毒性,通过抑制ROS产生抑制H_(2)O_(2)引起的B16-F10细胞损伤。The 4-butylresorcinol-containing cinnamon oil microemulsion-gel was prepared and its pharmaceutical properties were investigated.Its whitening and antioxidant properties were evaluated.Cinnamon oil microemulsion containing 4-butylresorcinol is clear and transparent in appearance.Its particle size is 15.68 nm and the coefficient of dispersity(PDI)is 0.185.The microemulsion-gel is prepared by using carbopol and Bletilla striata(T.)Reichb.F.polysaccharides as the gel matrix with the pH value at 6.0 and the viscosity at 74.8 Pa·s.In this study,the whitening effect of 4-butylresorcinol-containing cinnamon oil microemulsion-gel was determined by measuring the tyrosinase inhibition.It is shown that the tyrosinase inhibition of 4-butylresorcinol-loaded cinnamon oil microemulsion-gel gradually enhances with its concentration increasing.The tyrosinase inhibition activity of microemulsion-gels is higher than that of the free 4-butylresorcinol and arbutin at the same concentration.The antioxidant effect of 4-butylresorcinol-loaded cinnamon oil microemulsion-gel on hydrogen peroxide(H_(2)O_(2))-induced oxidative damage was tested in B16-F10 cells.The results show that microemulsion-gel has excellent antioxidant effect by reducing ROS production.

关 键 词：4-丁基间苯二酚 肉桂油 微乳凝胶 美白 抗氧化 

分 类 号：TQ658[化学工程—精细化工]