纤毛鹅观草-普通小麦高抗条锈病多年生属间杂种F1的创制及鉴定  

Generation and identification of a resistance to stripe rust perennial inter-generic hybrid F1 between Roegneria ciliaris and common wheat

作　　者：朱明昆 包俊浩 庞菁璐 周诗绮 方忠艳 郑文 张亚洲 吴丹丹[1,2] ZHU Ming-Kun;BAO Jun-Hao;PANG Jing-Lu;ZHOU Shi-Qi;FANG Zhong-Yan;ZHENG Wen;ZHANG Ya-Zhou;WU Dan-Dan(Triticeae Research Institute,Sichuan Agricultural University,Chengdu 611130,Sichuan,China;State Key Laboratory of Crop Gene Exploitation and Utilization in Southwest China,Chengdu 611130,Sichuan,China)

机构地区：[1]四川农业大学小麦研究所,四川成都611130 [2]西南作物基因资源发掘与利用国家重点实验室,四川成都611130

出　　处：《作物学报》2024年第6期1406-1419,共14页Acta Agronomica Sinica

基　　金：国家自然科学基金青年项目(32200180);四川省中央引导地方科技发展专项(2023ZYD0088);四川省教育厅项目(2022YFSY0035)资助。

摘　　要：本研究对鹅观草属(Roegneria C.Koch.)13个物种29份材料进行条锈病田间鉴定和抗条锈病基因检测,将筛选的抗病材料与小麦进行人工属间杂交,并对属间杂种F_(1)进行形态学、细胞遗传学及小麦条锈病抗性检测,结果显示:82.76%的材料在田间对条锈病表现中抗以上的抗性,且均含有5个以上的已知抗条锈病基因的等位基因,但仍可能存在条锈病抗性新基因;筛选到的一份高抗条锈病的纤毛鹅观草(Roegneria ciliaris[Trin.]Nevski)ZY11004-R,将其与5个普通小麦(Triticum aestivum L.)品种进行人工属间杂交,通过胚拯救的方式成功获得了纤毛鹅观草-普通小麦CSph2a属间杂种F_(1);杂种F_(1)体细胞染色体条数为35条(基因组组成为StYABD),花粉母细胞减数分裂I中期染色体多为单价体,形态特征处于两亲本之间,但在生活习性上获得多年生的性状,并表现出高抗条锈病。In this study,we evaluated the stripe rust resistance type and estimated the published resistance genes in 13 Roegneria species including 29 materials.Afterward,we conducted artificial crosses and investigated the morphology characters,genome constitution,and stripe rust resistance of the intergeneric hybrid F_(1)between stripe rust resistance Roegneria material and common wheat.The results showed as follows:82.76%of the 29 tested materials exhibited stripe rust resistance at adult stage,and they contained more than 5 alleles of stripe rust resistance genes,which might still carry new resistance genes to wheat stripe rust.An intergeneric hybrids R.ciliaris-CSph2a F_(1)was generated using the screened high stripe rust resistance Roegneria ciliaris[Trin.]Nevski(ZY11004-R)and com-mon wheat mutant CSph2a,and raised based on the embryo rescue technology.F_(1)hybrid contained 35 chromosomes with StYABD genome constitution and an average of 26.84 monovalents during pollen mother cell metaphase I.Besides,F_(1)exhibited morphological intermediacy,except the perennial living was inherited form from maternal R.ciliaris with high resistance to wheat stripe rust.

关 键 词：鹅观草属 条锈病 属间杂种 远缘杂交 多年生小麦 

分 类 号：S512.1[农业科学—作物学]