Bacillus velezensis G-1·脂肽悬浮种衣剂的制备及其对高粱丝黑穗病菌的防侵染效果  

Preparation of Bacillus velezensis G-1·lipopeptide suspension seed coating agent and its anti-infection effect on Sphacelotheca reiliana

作　　者：孙美红 王彩琴 王蓉[1] 白文斌 高振峰 SUN Mei-Hong;WANG Cai-Qin;WANG Rong;BAI Wen-Bin;GAO Zhen-Feng(Shanxi Agricultural University,Taiyuan 030031,Shanxi,China;Sorghum Research Institute,Shanxi Agricultural University,Yuci 030600,Shanxi,China)

机构地区：[1]山西农业大学,山西太原030031 [2]山西农业大学高粱研究所,山西榆次030600

出　　处：《作物学报》2024年第6期1467-1485,共19页Acta Agronomica Sinica

基　　金：中央引导地方科技发展资金项目(YDZJSX20231C012);山西省现代农业杂粮产业技术体系项目(2023CYJSTX03-16)资助。

摘　　要：为筛选出可有效抑制高粱丝黑穗病菌侵染高粱根部组织的生防微生物与天然产物,并在此基础上开发出便于在生产上推广应用的微生物悬浮种衣剂。本研究以晋杂2001号高粱为试验材料,选择试验室保藏的3株优秀抗病细菌和脂肽物质,首先通过种子萌发和活体侵染率测定试验明确了不同拮抗细菌和脂肽物质对高粱丝黑穗病菌的防侵染效果和较优使用浓度,随后以优选菌株和脂肽物质为活性成分进行了悬浮种衣剂配方优化、种衣剂较优使用量及贮藏稳定性研究,并在盆栽条件下测定了悬浮种衣剂对高粱丝黑穗病菌的防侵染效果。研究结果表明:Bacillus velezensis G-1及其次级代谢产物(脂肽)对高粱丝黑穗病菌侵染高粱根部组织具有良好抑制效果,较优使用浓度分别为1.0×10^(9)cfu mL^(–1)和600μg mL^(–1),且对晋杂2001号高粱种子萌发无显著抑制作用(P>0.05);B.velezensis G-1·脂肽悬浮种衣剂的较优配方为菌株G-1悬浮液(1.0×10^(11)cfu mL^(–1))5 mL、脂肽物质(8 mg mL^(–1))5 mL、羧甲基纤维素钠4 g、海藻酸钠0.6 g、分散剂MF 4 g、FeSO_(4)·7H_(2)O 0.2 g、MgSO_(4)·7H_(2)O 1 g、K_(2)HPO_(4)·3H_(2)O 0.5 g、色浆4 g,水补足100 g;B.velezensis G-1·脂肽悬浮种衣剂的较优使用量为25 g包装1 kg高粱种子,且对高粱丝黑穗病菌的盆栽侵染抑制率为87.74%;此外,B.velezensis G-1·脂肽悬浮种衣剂还对高粱幼苗生长表现出较好促生作用,并具有良好贮藏稳定性,常温下避光贮藏12个月后防侵染效果仅下降1.63%。说明B.velezensis G-1·脂肽悬浮种衣剂在高粱丝黑穗病绿色防控技术推广应用中具有良好应用前景。In order to screen for biocontrol microorganisms and natural products effective against Sphacelotheca reiliana infection in sorghum root tissue,and to develop a microbial suspension seed coating agent suitable for practical application in sorghum cultivation.In this study,Jinza 2001 sorghum and three excellent disease-resistant bacteria and natural product of lipopeptide were used as the experimental material.Firstly,the anti-infection effects and optimal concentrations of different disease-resistant bacte-ria and lipopeptide against S.reiliana were determined involving seed germination and in vivo infection rate determination.Sub-sequently,the optimal formulation,dosage,and storage stability of the suspension seed coating agent were studied based on the active ingredients of the optimum strain and lipopeptide.The anti-infection effect of suspended seed coating agent against S.rei-liana was measured under potted conditions.Results showed that Bacillus velezensis G-1 and its secondary metabolites(lipopep-tide)exhibited significant efficacy against S.reiliana on infection in sorghum root tissue,with optimal concentrations of 1.0×10^(9)cfu mL^(–1)for B.velezensis G-1 and and 600μg mL^(–1)lipopeptide,respectively.Moreover,there was no significant inhibitory effect on the germination of Jinza 2001 at the optimal concentrations of B.velezensis G-1 and lipopeptide(P>0.05).The optimal for-mula for the B.velezensis G-1·lipopeptide suspension seed coating agent comprised of 5 mL of strain G-1(1.0×10^(11)cfu mL^(–1)),5 mL of lipopeptide substance(8 mg mL^(–1)),4 g of sodium carboxymethyl cellulose,0.6 g of sodium alginate,4 g of dispersant MF,0.2 g of ferrous sulfate,1 g of magnesium sulfate,0.5 g of potassium dihydrogen phosphate,4 g of color paste,and water added to reach 100 g.The optimal dosage of B.velezensis G-1·lipopeptide suspension seed coating agent was 25 g packaged with 1 kg sorghum seeds,resulting in the anti-infection effect for S.reiliana infecting sorghum root was 87.74%under pot test conditions

关 键 词：高粱丝黑穗病菌 抗病微生物 脂肽 生物悬浮种衣剂 防侵染效果 

分 类 号：S435.14[农业科学—农业昆虫与害虫防治] TQ458[农业科学—植物保护]