外源褪黑素对盐胁迫下萝卜幼苗生长的影响  

Effects of exogenous melatonin on growth of radish seedlings under saltstress

作　　者：彭笑洁 崔玉莹 王浩[1] 张梓贺 武春成[1] 谢洋 PENG Xiaojie;CUI Yuying;WANG Hao;ZHANG Zihe;WU Chuncheng;XIE Yang(Hebei Key Laboratory of Horticultural Germplasm Excavation and Innovative Utilization/College of Horticulture Science and Technology,Hebei Normal University of Science and Technology,Qinhuangdao 066004,Hebei,China)

机构地区：[1]河北省特色园艺种质挖掘与创新利用重点实验室·河北科技师范学院园艺科技学院,河北秦皇岛066004

出　　处：《中国瓜菜》2024年第5期157-162,共6页China Cucurbits And Vegetables

基　　金：河北省现代农业产业技术体系建设专项资金(HBCT2024140205);2022年国家级大学生创新创业训练计划项目(202210798017)。

摘　　要：土壤盐渍化严重制约萝卜高效生产,褪黑素(melatonin,MT)作为天然抗氧化剂,在响应植物非生物胁迫中发挥重要作用。以满堂红萝卜为试验材料,分别采用浓度为0、50、100、150、200μmol·L^(-1)的MT进行喷施处理,筛选对萝卜生长最佳的MT浓度;采用基质盆栽,以200 mmol·L^(-1)Na Cl浇灌作为盐胁迫环境,以0μmol·L^(-1)MT(T1)和筛选出的最佳MT浓度(T2,150μmol·L^(-1))溶液喷施萝卜幼苗为处理组,非盐胁迫与未喷施MT作对照(CK)。结果表明,与CK相比,盐胁迫显著降低萝卜幼苗株高(定植后29 d除外)、茎粗、株幅、叶长、叶宽(定植后29 d除外)等指标,喷施150μmol·L^(-1)MT可提高定植25 d后的萝卜幼苗株高、茎粗、株幅、叶长、叶宽和叶绿素含量,增强SOD和CAT活性,降低MDA含量;其中定植后29 d,喷施150μmol·L^(-1)MT和0μmol·L^(-1)MT相比,株高、茎粗、叶长、叶宽和叶绿素含量分别提高了26.79%、28.16%、16.81%、29.11%、7.72%,SOD和CAT活性分别显著提高了32.12%和40.76%,MDA含量显著降低了38.92%,表明喷施150μmol·L^(-1)外源MT可有效缓解盐胁迫对萝卜幼苗的伤害,有效促进萝卜幼苗生长。Soil salinization seriously restricts the efficient production of radish.Melatonin(MT),as a natural antioxidant,plays an important role in responding to plant non-biological stress.Mantanghong radish was used as experimental material to be sprayed by MT with concentrations of 0,50,100,150 and 200μmol·L^(-1),respectively,to screen the best MT concentration for radish growth.Then,a matrix pot was used to irrigate radish with 200 mmol·L^(-1)NaCl as salt stress environment,spray radish with 0μmol·L^(-1)MT(T1)and the optimal MT concentration(T2)solution as treatment group,and control group(CK)was used between non-salt stress and non-spray MT.The results showed that compared with CK,salt stress significantly decreased the plant height(except 29 d after planting),stem diameter,leaf length and leaf width(except 29 d after planting)of radish seedlings.Spraying 150μmol·L^(-1)MT could increase the plant height,stem diameter,plant width,leaf length,leaf width and chlorophyll content of radish seedlings on 25 days after implantation.The activity of SOD and CAT was increased,and the content of MDA was decreased.On 29 days after implantation,compared with 150μmol·L^(-1)MT and 0μmol·L^(-1)MT,plant height,stem diameter,leaf length,leaf width and chlorophyll content increased by 26.79%,28.16%,16.81%,29.11%and 7.72%,respectively.SOD and CAT activities were significantly increased by 32.12%and 40.76%,respectively,and MDA content was significantly decreased by 38.92%,indicating that spraying 150μmol·L^(-1)exogenous MT could effectively alleviate the damage of salt stress on radish seedlings and promote the growth of radish seedlings.

关 键 词：萝卜 盐胁迫 褪黑素 幼苗生长 

分 类 号：S631.1[农业科学—蔬菜学]